In adults, the correspondence between chordotonal organs and staining pattern is good, but not perfect. With the exception of the Wheeler's organ, the pteropleura, and the tibia, (and the testis), all stained spots correspond to previously reported chordotonal organs. Two sites have the organs, but do not stain: the second antennal segment and the haltere.Expression in adult testis occurs in primary and secondary spermatocytes and perhaps in early postmeiotic spermatids.