FlyBase Allele Report: Dmel\Dfd[VP16.UAS]

General Information

Symbol

Dmel\Dfd^VP16.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0094851

Feature type

allele

Associated gene

Dmel\Dfd

Associated Insertion(s)

Carried in Construct

P{UAS-Dfd.T:VP16}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Li et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Dfd.T:VP16}

Cytology

Description

UASt regulatory sequences drive expression of the entire Dfd open reading frame tagged at the N-terminal end with Hsim\VP16 amino acids 413 to 490 (encodes the activation domain).

(Li et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dfd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^arm.PS

(Li et al., 1999)

some die during embryonic stage, with Scer\GAL4^arm.PS

(Li et al., 1999)

Phenotype Manifest In

cirri, with Scer\GAL4^arm.PS

(Li et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS

(Li et al., 1999)

embryonic segment, with Scer\GAL4^arm.PS

(Li et al., 1999)

eye, with Scer\GAL4^GMR.PF

(Lohmann et al., 2002)

mouth hook, with Scer\GAL4^arm.PS

(Li et al., 1999)

Detailed Description

Statement

Reference

When Dfd^{Scer\UAS.T:Hsim\VP16} is driven by Scer\GAL4^GMR.PF, a dramatically rough appearance and a severe reduction in the eye size is seen.

(Lohmann et al., 2002)

Embryos expressing Dfd^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^arm.PS have extreme cuticle defects along the entire anterior/posterior axis, whether the Scer\GAL4^arm.PS driver is provided maternally or paternally. These embryos are less than half the normal length, due to a dramatic narrowing and partial fusion of many body segments. Each of the rudimentary segments develops maxillary cirri and mouth hook fragments.

(Li et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Dfd^VP16.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Diap1^GMR.PH

(Lohmann et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

When th^GMR.PH is added to Dfd^{Scer\UAS.T:Hsim\VP16}, Scer\GAL4^GMR.PF animals the eye phenotype is suppressed.

(Lohmann et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dfd^{Scer\UAS.T:Hsim\VP16}

Dfd^UAS.T:VP16

Dfd^VP16.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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