FlyBase Allele Report: Dmel\Prosα2[SuDTS]

General Information

Symbol

Dmel\Prosα2^SuDTS

Species

D. melanogaster

Name

FlyBase ID

FBal0095585

Feature type

allele

Associated gene

Dmel\Prosα2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

Mutagen

ethyl nitrosourea

(Neuburger et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: C212Y.

(Neuburger et al., 2006)

Nucleotide substitution: G?A.

(Neuburger et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:12,432,394..12,432,394 [+]

Nucleotide change:

G12432394A

Reported nucleotide change:

G?A

Amino acid change:

C213Y | Prosalpha2-PA

Reported amino acid change:

C212Y

(Neuburger et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygotes show little if any embryonic lethality, but the lethal period is polyphasic throughout the larval and pupal stages. Some homozygous larvae show a slow growth rate and have sluggish behaviour, while others progress through the larval stages with normal appearance and behaviour, although pupation is usually delayed by a day or two. Most homozygous pupae fail to develop to late stages, although a few become pharate adults. No homozygotes eclose. Some dying homozygous larvae have necrotic gut tissue.

Pros25^SuDTS/Df(3R)T-32 animals do not reach the late larval or pupal stages. Mutant larvae show delayed development and sluggish behaviour.

Brains of homozygous larvae raised at 25^oC or 29^oC show no apparent polyploidy or aneuploidy and chromosome morphology and anaphase figures appear normal. The mean mitotic index is somewhat reduced compared to controls, probably reflecting the slower developmental rate of the mutant larvae.

(Neuburger et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Prosα2^SuDTS has lethal | recessive phenotype, suppressible by Pros26[+]/Prosβ6¹

(Neuburger et al., 2006)

NOT suppressed by

Statement

Reference

Prosα2^SuDTS has lethal | recessive phenotype, non-suppressible by Prosβ2¹/Prosbeta2[+]

(Neuburger et al., 2006)

Suppressor of

Statement

Reference

Prosα2^SuDTS/Su(DTS)-1[+] is a suppressor | partially of lethal | dominant | larval stage phenotype of Prosβ2¹/Prosbeta2[+], Prosβ6¹

(Neuburger et al., 2006)

Prosα2^SuDTS/Su(DTS)-1[+] is a suppressor | partially of lethal | dominant | larval stage phenotype of Prosβ2¹, Pros26[+]/Prosβ6¹

(Neuburger et al., 2006)

Prosα2^SuDTS/Su(DTS)-1[+] is a suppressor of lethal | dominant | heat sensitive phenotype of Prosβ2¹

(Neuburger et al., 2006)

Prosα2^SuDTS/Su(DTS)-1[+] is a suppressor of lethal | dominant | heat sensitive phenotype of Prosβ6¹

(Neuburger et al., 2006)

Prosα2^SuDTS/Su(DTS)-1[+] is a suppressor of lethal | recessive phenotype of Prosβ6¹

(Neuburger et al., 2006)

Prosα2^SuDTS/Su(DTS)-1[+] is a suppressor | partially of lethal | dominant | larval stage phenotype of Prosβ2¹, Prosβ6¹

(Smyth and Belote, 1999)

Other

Statement

Reference

Prosα2^SuDTS/Su(DTS)-1[+], Prosβ2¹ has viable phenotype

(Neuburger et al., 2006)

Prosα2^SuDTS/Su(DTS)-1[+], Prosβ6¹ has viable phenotype

(Neuburger et al., 2006)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Pros26¹ Pros25^SuDTS double heterozygotes are viable at 29^oC, in contrast to Pros26¹ single heterozygotes. The recessive lethality of Pros26¹ homozygotes seen at 25^oC is only partially suppressed by Pros25^SuDTS/+ and the survivors are slow developing and sick.

Prosβ2¹ Pros25^SuDTS double heterozygotes are viable at 29^oC, in contrast to Prosβ2¹ single heterozygotes.

Pros26¹ Prosβ2¹ double heterozygotes show early larval lethality at 25^oC, which is partially suppressed by Pros25^SuDTS/+.

Pros26¹/+ can weakly suppress the lethality of Pros25^SuDTS homozygotes at 25^oC.

(Neuburger et al., 2006)

Dominant suppressor of the dominant temperature sensitive lethal effect of Pros26¹ or Prosβ2¹. Pros26¹ and Prosβ2¹ are lethal in transheterozygous condition, even at normally permissive temperatures. This synthetic lethal effect is partially suppressed by Su(DTS)-1¹; a small fraction of + Pros26¹ Su(DTS)-1¹/Prosβ2¹ + + animals survive to adulthood at 18^oC.

(Smyth and Belote, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Prosα2^SuDTS is rescued by Prosα2^+t2

(Neuburger et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Induced on: A Pros26¹ chromosome.

(Neuburger et al., 2006)

Selected as: a suppressor of the dominant temperature sensitive lethality of Pros26¹.

(Neuburger et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Pros25^SuDTS

(Neuburger et al., 2006)

Prosα2^SuDTS

Su(DTS)

(Neuburger et al., 2006)

Su(DTS)-1

(Smyth and Belote, 1999)

Su(DTS)-1¹

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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