FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pka-C1W224R.UAS.Tag:FLAG
Open Close
General Information
Symbol
Dmel\Pka-C1W224R.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0098327
Feature type
allele
Associated gene
Dmel\Pka-C1
Associated Insertion(s)
Carried in Construct
P{UAS-Pka-C1.W224R.F}
Key Links
Transgenic product class
missense_variant
(Kiger et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kiger et al., 1999)
Mutagen
in vitro construct
(Kiger et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-Pka-C1.W224R.F}
Cytology
Description

UASt regulatory sequences drive expression of mutated cDNA of Pka-C1 (carries the amino acid replacement W224R). Also tagged with Tag:FLAG at C terminus. The mutated W224 is within a highly conserved region, thought to contribute to the stability of the catalytic loop and PKI peptide binding site.

(Kiger et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Pka-C1
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      All P{UAS-Pka-C1.W224R.F} insertions tested (1-2a, 1-3, 4-1, 5-1) are viable in the Scer\GAL4KO5, Scer\GAL4PP3, and Scer\GAL4JW1 backgrounds.

      (Kiger et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      Pka-C1W224R.UAS.Tag:FLAG is a non-suppressor of lethal phenotype of Ocun\PKIUAS.Tag:FLAG, Scer\GAL4RK5

      (Kiger et al., 1999)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pka-C1W224R.Scer\UAS.T:Zzzz\FLAG
      Pka-C1W224R.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)