FlyBase Allele Report: Dmel\Mhc[R57-24.Act88F]

General Information

Symbol

Dmel\Mhc^{R57-24.Act88F}

Species

D. melanogaster

Name

FlyBase ID

FBal0098370

Feature type

allele

Associated gene

Dmel\Mhc

Associated Insertion(s)

Carried in Construct

P{Act88F-Mhc.R57-24}

Also Known As

Y97

Key Links

Transgenic product class

inframe_deletion

(Cripps et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Cripps et al., 1999)

Mutagen

in vitro construct

(Cripps et al., 1999)

Progenitor genotype

Carried in construct

P{Act88F-Mhc.R57-24}

Cytology

Description

Construct: The 5' regulatory region, first exon, first intron and translational start site of Act88F are fused upstream of cDNA/genomic sequences encoding the major adult form of Mhc lacking the globular head region. The Mhc coding sequences extend from 45 amino acids upstream of the head-rod junction through to the C-terminus. Exon 15a is included and a splicing choice for exon 18 is retained. A 1.5kb fragment from the 3' region of Mhc (which includes polyadenylation signals) is included downstream of the Mhc coding sequences.

(Cripps et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

Act88F

Encoded product / tool

Mhc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight

(Cripps et al., 1999)

flightless

(Nongthomba et al., 2003)

flightless, with Mhc¹⁰

(Nongthomba et al., 2003)

visible

(Nongthomba et al., 2003)

visible, with Mhc¹⁰

(Nongthomba et al., 2003)

Phenotype Manifest In

indirect flight muscle & myofibril

(Cripps et al., 1999)

indirect flight muscle & sarcomere

(Cripps et al., 1999)

indirect flight muscle & striated muscle thick filament

(Cripps et al., 1999)

indirect flight muscle & Z disc

(Cripps et al., 1999)

indirect flight muscle cell

(Cripps et al., 1999)

wing

(Nongthomba et al., 2003)

wing, with Mhc¹⁰

(Nongthomba et al., 2003)

Detailed Description

Statement

Reference

56% of homozygous adults have an upheld wing phenotype, 30% have their wings held down and 14% hold their wings in the normal position. The indirect flight muscle fibres appear normal. 18% of heterozygous adults have an have their wings held down and 82% hold their wings in the normal position. The indirect flight muscle fibres appear normal. 44% of Mhc¹⁰/+ ; Mhc^{R57-24.Act88F}/+ flies have their wings held down and 56% hold their wings in the normal position. The indirect flight muscle fibres appear normal.

(Nongthomba et al., 2003)

Flies carrying Mhc^{R57-24.Act88F} show reduced flight ability compared to controls. The degree of flight impairment correlates well with the level of Mhc^{R57-24.Act88F} protein accumulation. Thick and thin filaments are present in pupal indirect flight muscles (IFMs) of flies carrying Mhc^{R57-24.Act88F}, but myofibrils are structurally defective. Sarcomeres form although frequent cracks are seen in the myofilament lattice and sarcomere length and width are more variable than wild type. There are defects in the packing of thick filaments and the myofibrils are frequently not round in cross-section. Average sarcomere length is normal. Myofibril diameter is increased by approximately 20%. The number of thick filaments/cross-sectional area is reduced by approximately 40%. Mhc¹⁰ flies expressing Mhc^{R57-24.Act88F} have thick filaments that are associated with myofibril-like structures in the IFMs. These myofibrils have Z-discs and occasional M-lines, however, sarcomere length and the length of the thick filaments is variable. The Z-discs are thicker than wild type. Many of the thick filaments are packed in a hexagonal manner and are frequently interdigitated with thin filaments. Often, six thin filaments surround each thick filament, as in wild type. However, the myofibrils are rarely circular in cross-section and show a variability in size compared to wild type.

(Cripps et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor of visible phenotype of up¹

(Nongthomba et al., 2007)

Mhc[+]/Mhc^{R57-24.Act88F} is a suppressor of visible phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor | partially of visible phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

NOT Suppressor of

Statement

Reference

Mhc[+]/Mhc^{R57-24.Act88F} is a non-suppressor of flightless phenotype of up¹⁰¹

(Nongthomba et al., 2003)

Mhc[+]/Mhc^{R57-24.Act88F} is a non-suppressor of visible phenotype of up¹⁰¹

(Nongthomba et al., 2003)

Mhc[+]/Mhc^{R57-24.Act88F} is a non-suppressor of flightless phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a non-suppressor of flightless phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor of wing phenotype of up¹

(Nongthomba et al., 2007)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor | partially of indirect flight muscle cell phenotype of up¹

(Nongthomba et al., 2007)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor | partially of myosin filament & indirect flight muscle phenotype of up¹

(Nongthomba et al., 2007)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor | partially of striated muscle thin filament & indirect flight muscle phenotype of up¹

(Nongthomba et al., 2007)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor | partially of Z disc & indirect flight muscle phenotype of up¹

(Nongthomba et al., 2007)

Mhc[+]/Mhc^{R57-24.Act88F} is a suppressor of indirect flight muscle cell phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Mhc[+]/Mhc^{R57-24.Act88F} is a suppressor of wing phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor | partially of indirect flight muscle cell phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Mhc^{R57-24.Act88F}/Mhc¹⁰ is a suppressor | partially of wing phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

NOT Suppressor of

Statement

Reference

Mhc[+]/Mhc^{R57-24.Act88F} is a non-suppressor of wing phenotype of up¹⁰¹

(Nongthomba et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

up¹/Y ; Mhc¹⁰/Mhc⁺ ; Mhc^{R57-24.Act88F} flies have a normal wing posture and a normal indirect flight muscle morphology under polarised light. Ultrastructurally, sarcomere-like assemblies with Z-bands and thick and thin filaments are seen, although in most areas the filaments appear disorganised.

(Nongthomba et al., 2007)

Homozygous Mhc¹⁰ flies expressing Mhc^{R57-24.Act88F} completely restore indirect flight muscle fibre defects to a normal structural condition in heterozygous wupA^hdp-3 flies. The thorax of wupA^hdp-3/Y; Mhc¹⁰/+ flies have a small bunch of visible muscle mass, in contrast to wupA^hdp-3 ; Mhc+/+ flies which have no visible indirect flight muscle. wupA^hdp-3/Y; Mhc¹⁰/+ flies that express one copy of Mhc^{R57-24.Act88F} show a greater increase in muscle mass. This increase is greater still when flies express two copies of Mhc^{R57-24.Act88F}. Only in wupA^hdp-3/Y; Mhc¹⁰/Mhc¹⁰ flies that express two copies of Mhc^{R57-24.Act88F} can actual myofibrillar structures be seen. In wupA^hdp-3/Y; Mhc¹⁰/Mhc¹⁰; Mhc^{R57-24.Act88F} flies thick filaments, some thin filaments, isolated Z-discs and tiger-tails (formed by serially repeated Z-discs connected by short stretches of thin filaments) are readily visible but sarcomeric structures fail to assemble.

(Nongthomba et al., 2004)

The defects seen in wupA^hdp-2/Y flies are partially suppressed by Mhc^{R57-24.Act88F}/+; 51% of the double mutant adults have an upheld wing phenotype, 39% have their wings held down and 10% hold their wings in the normal position. 61% of the indirect flight muscle fibres are hypercontracted, 39% show a partial hypercontraction phenotype. The defects seen in wupA^hdp-2/Y flies are almost completely suppressed by Mhc¹⁰/+ ; Mhc^{R57-24.Act88F}/+ - 13% of the double mutant adults have an upheld wing phenotype, 0% have their wings held down and 87% hold their wings in the normal position. 13% of the indirect flight muscle fibres show a partial hypercontraction phenotype and 87% are normal. The wing phenotypes of up¹⁰¹/Y flies are not suppressed by Mhc^{R57-24.Act88F}/+, while in the double mutants, 50% of the indirect flight muscle fibres are hypercontracted, 50% show a partial hypercontraction phenotype.

(Nongthomba et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Mhc^{R57-24.Act88F} partially rescues Mhc¹³/Mhc¹⁰

(Nongthomba et al., 2003)

Mhc^{R57-24.Act88F} partially rescues Mhc¹⁰

(Cripps et al., 1999)

Comments

Some of the ultrastructural defects of Mhc¹⁰ indirect flight muscles are rescued by Mhc^{R57-24.Act88F}, but flight ability is not rescued.

(Cripps et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mhc^R21-1.Act88F has a more severe effect on flight ability than Mhc^{R57-24.Act88F}.

(Cripps et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mhc^{R57-24.Act88F}

Y97

(Nongthomba et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (9)

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