FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\MhcR21-1.Act88F
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General Information
Symbol
Dmel\MhcR21-1.Act88F
Species
D. melanogaster
Name
FlyBase ID
FBal0098371
Feature type
allele
Associated gene
Dmel\Mhc
Associated Insertion(s)
Carried in Construct
P{Act88F-Mhc.R21-1}
Key Links
Transgenic product class
inframe_deletion
(Cripps et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Cripps et al., 1999)
Mutagen
in vitro construct
(Cripps et al., 1999)
Progenitor genotype
Carried in construct
P{Act88F-Mhc.R21-1}
Cytology
Description

Construct: The 5' regulatory region, first exon, first intron and translational start site of Act88F are fused upstream of cDNA/genomic sequences encoding an embryonic form of Mhc lacking the globular head region. The Mhc coding sequences extend from 45 amino acids upstream of the head-rod junction through to the C-terminus. Exon 15b is included and is prespliced at the 3' end such that exon 18 is excluded (this alters the amino acid sequence at the C-terminus so that it matches the embryonic isoform). A 1.5kb fragment from the 3' region of Mhc (which includes polyadenylation signals) is included downstream of the Mhc coding sequences.

(Cripps et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
Act88F
Act88F_2kb
Encoded product / tool
Mhc
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      indirect flight muscle & myofibril

      (Cripps et al., 1999)

      indirect flight muscle & sarcomere

      (Cripps et al., 1999)

      indirect flight muscle & striated muscle thick filament

      (Cripps et al., 1999)

      indirect flight muscle & Z disc

      (Cripps et al., 1999)

      indirect flight muscle cell

      (Cripps et al., 1999)
      Detailed Description
      Statement
      Reference

      Flies carrying MhcR21-1.Act88F show reduced flight ability compared to controls. The degree of flight impairment correlates well with the level of MhcR21-1.Act88F protein accumulation. Thick and thin filaments are present in pupal indirect flight muscles (IFMs) of flies carrying MhcR21-1.Act88F, but myofibrils are structurally defective. Sarcomeres form although frequent cracks are seen in the myofilament lattice and sarcomere length and width are more variable than wild type. There are defects in the packing of thick filaments and the myofibrils are frequently not round in cross-section. Average sarcomere length is significantly reduced compared to wild type. Myofibril diameter is increased by approximately 20%. The number of thick filaments/cross-sectional area is reduced by approximately 40%. Mhc10 flies expressing MhcR21-1.Act88F have hollow thick filaments and myofibril-like structures in the IFMs. Hexagonal packing of the thick filaments with arrays of six thin filaments surrounding each thick filament is seen, as in wild type. Less rescue is seen than with MhcR57-24.Act88F.

      (Cripps et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      MhcR21-1.Act88F partially rescues Mhc10

      (Cripps et al., 1999)
      Comments

      Some of the ultrastructural defects of Mhc10 indirect flight muscles are rescued by MhcR21-1.Act88F, but flight ability is not rescued.

      (Cripps et al., 1999)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      MhcR21-1.Act88F has a more severe effect on flight ability than MhcR57-24.Act88F.

      (Cripps et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      MhcR21-1.Act88F
      Name Synonyms
      Secondary FlyBase IDs
        References (3)