FlyBase Allele Report: Dmel\Mef2[III.UAS]

General Information

Symbol

Dmel\Mef2^III.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0101611

Feature type

allele

Associated gene

Dmel\Mef2

Associated Insertion(s)

Carried in Construct

P{UAS-Mef2.III}

Also Known As

UAS-Mef2

Key Links

Transgenic product class

wild_type

(Gunthorpe et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Gunthorpe et al., 1999)

Mutagen

in vitro construct

(Gunthorpe et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Mef2.III}

Cytology

Description

UAS regulatory sequences drive expression of the type III Mef2 isoform.

(Gunthorpe et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mef2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^P0.5.Pdf

(Blanchard et al., 2010)

abnormal locomotor rhythm, with Scer\GAL4^tim.UAS

(Blanchard et al., 2010)

Phenotype Manifest In

embryo | dorsal closure stage, with Scer\GAL4^da.G32

(Gunthorpe et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^da.G32

(Gunthorpe et al., 1999)

ventral denticle belt, with Scer\GAL4^da.G32

(Gunthorpe et al., 1999)

Detailed Description

Statement

Reference

Expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^P0.5.Pdf causes long locomotor activity rhythms, with a period of 25.1 +/- 0.1 hours.

Expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^tim.Scer\UAS causes long locomotor activity rhythms, with a period of 24.6 +/- 0.3 hours. 61.5% of the flies have a single rhythm, 23.1% of flies have a complex rhythm (classified as more than one rhythmic period detectable above the 95% significance line using Lomb-Scargle analysis of periodograms) and 15.4% are arrhythmic.

(Blanchard et al., 2010)

Dorsal closure fails in embryos expressing Mef2^III.Scer\UAS under the control of Scer\GAL4^da.G32. The cuticle is thin but has denticle belts, although their pattern is abnormal. Expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^twi.PB results in embryos that are phenotypically wild type.

(Gunthorpe et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^twi.PB/Mef2^III.UAS is a non-suppressor of embryonic myoblast phenotype of lmd^A388

(Ruiz-Gomez et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^how-24B/Mef2^III.UAS rescues Mef2^22-21

(Gunthorpe et al., 1999)

Comments

At 18^oC, expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^how-24B rescues midgut constrictions in 90% of Mef2^22-21 embryos. The pharyngeal muscles are wild-type in 50% of the embryos. A subset of muscles, including some dorsal (dorsal transverse muscle 1), some lateral (lateral longitudinal muscle 1, segment border muscle) and some ventral muscles (ventral transverse muscle 1, ventral acute muscle 1, ventral acute muscle 2) are rescued. Unfused myoblasts are seen in the majority of hemisegments. The heart shows a range of phenotypes in these embryos. At 25^oC, expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^how-24B rescues midgut constrictions in Mef2^22-21 embryos. The shape of the pharyngeal muscle cells is not wild type in 30% of embryos, while it is fully rescued in other embryos. The somatic musculature is almost rescued to wild type, although occasionally one or two muscles in a hemisegment are absent or have an abnormal shape. The variety of phenotypes seen in the heart is similar to that seen when Mef2^III.Scer\UAS is expressed under the control of Scer\GAL4^how-24B at 18^oC. At 29^oC, when Mef2^III.Scer\UAS is expressed under the control of Scer\GAL4^how-24B in Mef2^22-21 embryos there are no midgut constrictions. The pharyngeal muscles are completely rescued. The heart is disorganised posteriorly in some embryos. The somatic musculature is fully rescued; the pattern of myosin-expressing fibres is essentially wild type, although there are some unfused myoblasts in the majority of hemisegments.

(Gunthorpe et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mef2^III.Scer\UAS

Mef2^III.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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