Microtubule motility is dramatically decreased in neurons from maternal Khc²³/Khc²³ (Khc²³ germline clone) and zygotic Khc²³/Khc²⁷ embryos. Most of the neurons cultured from these embryos die after overnight culture.

Khc²³/Khc²⁷ larvae show reduced flux of neurosecretory dense core vesicles along the axons compared to wild type.

Results in larval posterior paralysis in combination with a null Khc allele.

Both anterograde and retrograde flux of axonal mitochondria is inhibited in Khc²³/Khc²⁷ larvae.

45% of eggs from females containing homozygous germline clones show defects in dorsal appendage morphology; 38% have fused dorsal appendages, 5% have reduced dorsal appendages and 2% have missing dorsal appendages.

The velocity of ooplasmic streaming in stage 10B oocytes is dramatically reduced in mutant females compared to wild type.

Approximately 85% of Khc²³ mutants are embryonic lethal, with the remaining 15% lethal in the larval/pupal stages. Endosomes in Khc²³ stage 9 embryos exhibit saltations, but no not exhibit slow-streaming currents and have an approximate 1.7-fold reduction in mean velocity compared to wild-type. In stage 10B-11 Khc²³ oocytes, saltation and some short, individual dsiplacements occur, although concerted fast currents are rare, occuring in a small region of only one out of nine oocytes. Khc²³ endosomes move at a mean velocity approximately 11-fold less than wild-type.

Hemizygotes do not survive to adulthood.