A StuI site has been created at the 5' end of the Act88F coding sequence and an EcoRI site has been created at the 3' end.
The indirect flight muscles of flies expressing Act88FStuI.EcoRI in a Act88F6 background show highly ordered sarcomeres marked by Z-discs and M-lines and myofibrils are wild-type in appearance.
Act88FStuI.EcoRI rescues Act88F6