MARCM-mediated expression of lola4.7.Scer\UAS, using Scer\GAL4GH146, results in strong targeting defects in antero-dorsal projection neuron (adPN) and lateral (lPN) neuroblast clones. Phenotypes often include a large increase in wandering dendrites in the antennal lobe (AL) and some degree of loss of targeting and ectopic targeting. Often dendrites appear to be largely restricted to one region of the AL, such as dorsal or lateral, and have wandering dendrites through this region in many glomeruli, but fail to innervate normal targets or extend any dendrites into other regions of the AL.
vPN neuroblast MARCM clones expressing lola4.7.Scer\UAS, using Scer\GAL4GH146, show a loss of targeting to DA1 and the frequent extension of dendrites outside of the AL boundary, most often in the ventral region projecting towards the suboesophageal ganglion. Processes of the pan-AL vPN are not elaborated properly and only partially innervate the AL.
MARCM-mediated expression of lola4.7.Scer\UAS, using Scer\GAL4GH146, results in variable DL1 phenotypes, ranging from a complete loss of DL1 innervation and targeting to other regions of the AL, to DL1 targeting with dendritic extensions outside of the DL1 glomerular region. Axon targeting is also affected, most often resulting in a high degree of ectopic branching, axon bifurcation and incorrect lateral horn innervation patterns.
MARCM-mediated expression of lola4.7.Scer\UAS, using Scer\GAL4GH146, results in few dendritic phenotypes at 18[o]C, while at 25[o]C and 29[o]C dendrites mistarget and have ectopic innervations and axons have additional branches and bifurcations.
MARCM clones simultaneously mutant for lolae76 and lola4.7.Scer\UAS, induced using Scer\GAL4GH146, exhibit: i) cell loss and lack of dendritic extensions in adPN and lPN neuroblast clones; ii) a strong reduction of VA1lm and DA1 targeting in vPN clones, with the pan-AL neuron often showing little dendritic process elaboration and only rare innervation of much of the AL.
DL1 MARCM clones simultaneously mutant for lolae76 and lola4.7.Scer\UAS, induced using Scer\GAL4GH146 either fail to innervate DL1 or have extensions into other regions of the AL. Axonal defects range from a failure to extend or elaborate branches in the LH to an increase in ectopic branching.