FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Klp64Dk33
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General Information
Symbol
Dmel\Klp64Dk33
Species
D. melanogaster
Name
FlyBase ID
FBal0102964
Feature type
allele
Associated gene
Dmel\Klp64D
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ray et al., 1999)
Progenitor genotype
Cytology
Description

Nucleotide substitution: T?G in the 3' untranslated region 4bp after the predicted normal stop codon.

(Ray et al., 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:5,357,217..5,357,217 [-]
Nucleotide change:

T5357217G

Reported nucleotide change:

T?G

Comment:

Reported as a T to G nucleotide change four bases after the stop codon.

(Ray et al., 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      About 18% of Klp64Dk33 homozygous first instar larvae from Klp64Dk33 heterozygous parents show a weak segment polarity phenotype with additional ectopic denticles in the abdominal segments A4-A7.

      (Vuong et al., 2014)

      Homozygotes die at or before the early third larval instar stage in a typical crowded culture. If the homozygotes are raised in low density conditions without competition from wild-type larvae, a few mutant animals survive to be pharate pupae and occasionally eclose as fully formed adults. The adults show an acutely uncoordinated walk, are unable to stand and die in a few hours. Mutant third instar larvae show slight to severe sluggishness and roll abnormally from side to side during crawling.

      (Ray et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Phenotype Manifest In
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Approximately 20% of embryos double heterozygous for Klp64Dk33 and arm1 exhibit ectopic denticles in the space between normal denticle belts. The phenotype is more severe than is seen in Klp64Dk33 homozygotes. Klp64Dk33/+; arm1/+ embryos at stage 10 show segment polarity phenotypes in the posterior region. The anterior region shows loss of cuticle due to incomplete cellularization.

      (Vuong et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Klp64Dn123

      (Ray et al., 1999)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Klp64Dk33
      (Vuong et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)