Construct: Contains a 4kb genomic NotI fragment. The 5' end of nod is also included.
A 4.0kb NotI rescue fragment is reported. The downstream NotI site is absent from the reference genomic sequence; as a result, the annotated endpoint of the rescue fragment is approximate; position of restriction fragment on reference sequence inferred by FlyBase curator.
Chromosomal decondensation surrounding autosomal insertions of P{GMroX2} is seen in polytene chromosomes if E(bx)Nurf301-3 is introduced into a roX1- roX2- background (roX1ex6 Df(1)52 P{w+4Δ4.3}) carrying P{GMroX2} insertions.
The male lethality caused by the roX1ex6 Df(1)52 P{w+4Δ4.3} combination is rescued with an average frequency of 82% by roX2t4.0.