FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Mmus\Dnmt3aUAS.cLa
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General Information
Symbol
Mmus\Dnmt3aUAS.cLa
Species
M. musculus
Name
Saccharomyces cerevisiae UAS construct a of Lyko
FlyBase ID
FBal0104085
Feature type
allele
Associated gene
Mmus\Dnmt3a
Associated Insertion(s)
Carried in Construct
P{UAS-Mmus\Dnmt3a.L}
Transgenic product class
cDNA
(Lyko et al., 1999)
wild_type
(Lyko et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lyko et al., 1999)
wild_type
(Lyko et al., 1999)
Mutagen
in vitro construct
(Lyko et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-Mmus\Dnmt3a.L}
Cytology
Description

UASt regulatory sequences drive expression of a Mmus\Dnmt3a cDNA.

(Lyko et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mmus\Dnmt3a
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Mmus\Dnmt3aScer\UAS.cLa under the control of Scer\GAL4da.G32 results in a significant reduction in cell number in third larval instar wing discs. The mitotic index is slightly elevated and BrdU incorporation is reduced in metaphase and anaphase chromosomes of third larval instar brain neuroblasts. The salivary glands are reduced in size compared to controls. Salivary gland nuclei sometimes show rudimentary polytenisation, but in most cases the DNA appears similar to interphase nuclei with no obvious polytenisation.

      (Weissmann et al., 2003)

      Flies expressing Mmus\Dnmt3aScer\UAS.cLa under the control of Scer\GAL41032.hx generally die during pupal development. Some flies eclose partially and die within one day. Others eclose fully and are viable, but have etched tergites with missing cuticle. Flies expressing Mmus\Dnmt3aScer\UAS.cLa under the control of Scer\GAL4ey.PS have small or absent eyes.

      (Lyko et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      Mmus\Dnmt3aUAS.cLa, Scer\GAL41032.hx has lethal phenotype, suppressible | partially by Su(var)3-9unspecified/Su(var)3-9[+]

      (Weissmann et al., 2003)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Mmus\Dnmt3aUAS.cLa, Scer\GAL41032.hx has polytene chromosome phenotype, suppressible by Su(var)3-9unspecified/Su(var)3-9[+]

      (Weissmann et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Su(var)3-9unspecified/+ partially suppresses the lethality caused by expression of Mmus\Dnmt3aScer\UAS.cLa under the control of Scer\GAL41032.hx. The rescued adults are viable and fertile with a normal appearance. A large number of larvae expressing Mmus\Dnmt3aScer\UAS.cLa under the control of Scer\GAL41032.hx in a Su(var)3-9unspecified/+ background have polytene chromosomes that are similar to those of controls.

      (Weissmann et al., 2003)

      Co-expression of Mmus\DnmtScer\UAS.cLa enhances the phenotype of flies expressing Mmus\Dnmt3aScer\UAS.cLa under the control of Scer\GAL41032.hx; flies generally die during early, undifferentiated pupal stages.

      (Lyko et al., 1999)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Mmus\Dnmt3aScer\UAS.cLa
      Mmus\Dnmt3aUAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Lyko
      (Lyko et al., 1999)
      Secondary FlyBase IDs
        References (2)