FlyBase Allele Report: Dmel\chb[P4]

General Information

Symbol

Dmel\chb^P4

Species

D. melanogaster

Name

FlyBase ID

FBal0118300

Feature type

allele

Associated gene

Dmel\chb

Associated Insertion(s)

Carried in Construct

Also Known As

mast^P4

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Lemos et al., 2000)

Progenitor genotype

chb^v40

(Lemos et al., 2000)

Cytology

Description

A deletion of up to 1kb of genomic sequence downstream of the P{lArB}chb^v40 insertion site. The insertion itself is incomplete lacking the ry gene.

(Lemos et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Lemos et al., 2000)

abnormal neuroanatomy

(Lowery et al., 2010, Lee et al., 2004)

lethal - all die before end of pupal stage | recessive

(Lemos et al., 2000)

some die during pupal stage | recessive

(Lemos et al., 2000)

Phenotype Manifest In

fascicle

(Lowery et al., 2010)

mitotic cell cycle

(Lemos et al., 2000)

presumptive embryonic/larval central nervous system

(Lee et al., 2004)

Detailed Description

Statement

Reference

chb^P4 mutant embryos exhibit a moderate penetrance (38%) of ectopic midline crossing.

(Lowery et al., 2010)

Homozygous embryos show ectopic crossing of the midline by axons in the central nervous system.

(Lee et al., 2004)

Third instar larval brains exhibit polyploidy and circular mitotic figures. Most cells show highly condensed chromatin. The most severe cases, cells show extensive polyploidy wit most chromosomes organised in a sphere-like conformation. Mutants have a mitotic index that is several times larger than seen in wild-type (9.3 compared to 1.14), almost all of which are arrested in metaphase or prometaphase. Circular mitotic figures are also seen.

(Lemos et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

chb^P4 has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^insc-Mz1407/msps^d03376

(Lowery et al., 2010)

chb^P4 has abnormal neuroanatomy phenotype, enhanceable by Abl²

(Lee et al., 2004)

chb^P4 has abnormal neuroanatomy phenotype, enhanceable by Abl⁴

(Lee et al., 2004)

Enhancer of

Statement

Reference

chb^P4 is an enhancer of abnormal neuroanatomy phenotype of Abl²

(Lee et al., 2004)

chb^P4 is an enhancer of abnormal neuroanatomy phenotype of Abl⁴

(Lee et al., 2004)

Other

Statement

Reference

chb^P4, sli²/sli[+] has abnormal neuroanatomy phenotype

(Lee et al., 2004)

chb^P4, sli² has abnormal neuroanatomy phenotype

(Lee et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

chb^P4 has fascicle phenotype, enhanceable by Scer\GAL4^insc-Mz1407/msps^d03376

(Lowery et al., 2010)

chb^P4 has larval neuron phenotype, enhanceable by Abl²

(Lee et al., 2004)

chb^P4 has larval neuron phenotype, enhanceable by Abl⁴

(Lee et al., 2004)

Enhancer of

Statement

Reference

chb^P4 is an enhancer of larval neuron phenotype of Abl²

(Lee et al., 2004)

chb^P4 is an enhancer of larval neuron phenotype of Abl⁴

(Lee et al., 2004)

Other

Statement

Reference

chb^P4, sli²/sli[+] has larval neuron phenotype

(Lee et al., 2004)

chb^P4, sli² has larval neuron phenotype

(Lee et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of msps^d03376 in the nervous system under the control of Scer\GAL4¹⁴⁰⁷ in a chb^P4 mutant background generate a severe ectopic midline crossing defect, often affecting numerous segments.

(Lowery et al., 2010)

The frequency of axons ectopically crossing the midline in chb^P4 Abl⁴ double homozygous embryos is substantially increased compared to the frequency seen in either single homozygote. The frequency of axons ectopically crossing the midline in chb^P4 Abl² double homozygous embryos is substantially increased compared to the frequency seen in either single homozygote. sli² chb^P4 double heterozygous embryos have axons ectopically crossing the midline. capt¹⁰ chb^P4 double heterozygous embryos do not show a significant increase in axons ectopically crossing the midline compared to controls.

(Lee et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The effects on viability, mitotic phenotype and mitotic progression suggest an allelic series: chb^EP3515 < chb^v40 < chb^EP3403 < chb^P4.

(Lemos et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

chb^P4

mast4

(Lemos et al., 2000)

mast^P4

(Lowery et al., 2010, Lee et al., 2004, Lemos et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close