FlyBase Allele Report: Dmel\tum[R417Q.UAS]

General Information

Symbol

Dmel\tum^R417Q.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0122005

Feature type

allele

Associated gene

Dmel\tum

Associated Insertion(s)

Carried in Construct

P{UAS-RacGAP.R417Q}

Key Links

Transgenic product class

dominant_negative_variant

(Sotillos and Campuzano, 2000)

missense_variant

(Sotillos and Campuzano, 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Sotillos and Campuzano, 2000)

missense_variant

(Sotillos and Campuzano, 2000)

Mutagen

in vitro construct

(Sotillos and Campuzano, 2000)

Progenitor genotype

Carried in construct

P{UAS-RacGAP.R417Q}

Cytology

Description

UASt governs the expression of a dominant negative form of tum cDNA (carries the amino acid replacement R417Q).

(Sotillos and Campuzano, 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tum

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

abnormal planar polarity, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

abnormal planar polarity, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

visible, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

visible, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

visible, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Phenotype Manifest In

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing & cell, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing & cell, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing & cell, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing & trichome, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing & trichome, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing & trichome, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Detailed Description

Statement

Reference

When expression is driven by Scer\GAL4^en-e16E wings are notched, wing veins are increased in width and can be fused and extra wing vein material appears connecting neighboring veins. Extra campaniform sensillae develop in association with wing veins L3, L4 and L5. Cells are enlarged (visible as early as the imaginal disc), and trichomes show altered polarity and number. When expression is driven by Scer\GAL4^Bx-MS1096 or Scer\GAL4^69B the result is the same.

(Sotillos and Campuzano, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

606115

w^*; P{UAS-RacGAP.R417Q}3

Notes on Origin

Discoverer

Comments

Effect weaker than for RacGAP50C^{ΔE1E.Scer\UAS}.

(Sotillos and Campuzano, 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

RacGAP50C^{P417Q.Scer\UAS}

RacGAP^{P417Q.Scer\UAS}

(Sotillos and Campuzano, 2000)

tum^{P417Q.Scer\UAS}

tum^P417Q.UAS

tum^R417Q.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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