The number of repo-expressing longitudinal glia (LG) cells is reduced in mutant embryos, although their location is initially relatively normal. Glial migration and the initial orientation of pioneer neurons are not affected until stage 13. At stage 14, some glial cells migrate to abnormal positions; some are located laterally and are associated with motor nerves where segmental or intersegmental nerve root glia reside at stage 15. These defects occur in 40% of abdominal hemisegments. These mislocated glial cells are likely to be LG that have migrated abnormally because of their characteristic round nuclei and their expression of an LG-specific marker. Longitudinal connectives are frequently disrupted in segments where LG have migrated abnormally. Defects in formation of the dMP2 fascicle are seen in the hemisegments where glial cells are missing and dMP2 pathfinding errors are seen where glial cells are mislocated.