FlyBase Allele Report: Dmel\Ras85D[D38E]

General Information

Symbol

Dmel\Ras85D^D38E

Species

D. melanogaster

Name

FlyBase ID

FBal0123121

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{Ras85D^D38E}

Key Links

Transgenic product class

missense_variant

(Halfar et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Halfar et al., 2001)

Mutagen

in vitro construct

(Halfar et al., 2001)

Progenitor genotype

Ras85D^+t3.6

(Halfar et al., 2001)

Carried in construct

P{Ras85D^D38E}

Cytology

Description

Construct: Amino acid replacement: D38E.

(Halfar et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

Ras85D

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | somatic clone, with Ras85D^x7b

(Halfar et al., 2001)

Phenotype Manifest In

eye | somatic clone, with Ras85D^x7b

(Halfar et al., 2001)

Detailed Description

Statement

Reference

Introduction of Ras85D^D38E into flies that are homozygous for Ras85D^x7b in the eye disc results in a slight enlargement of the eye field, due to the presence of unpigmented cuticle, but no additional ommatidial structures are formed (compared to flies that are homozygous for Ras85D^x7b in the eye disc that are not also carrying Ras85D^D38E).

(Halfar et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^D38E, Ras85D^x7b has visible | somatic clone phenotype, suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^D38E, Ras85D^x7b has adult head | somatic clone phenotype, suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

Ras85D^D38E, Ras85D^x7b has eye | somatic clone phenotype, suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

rl^Sem/+ alone is not sufficient to rescue the reduced eye and head phenotypes of flies that are homozygous for Ras85D^x7b in the eye disc. However, the addition of Ras85D^D38E in combination with rl^Sem results in a substantial rescue of the Ras85D^x7b eye and head size phenotypes; differentiated photoreceptor cells are seen and a substantial fraction of the ommatidia contain the full complement of photoreceptors.

(Halfar et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Ras85D^D38E partially rescues Ras85D^x7b

(Halfar et al., 2001)

Comments

Fails to rescue Ras85D^x7b to adulthood. Partially rescues the growth defects of Ras85D^x7b clones in the eye disc. In addition, the rescued cells survive beyond the morphogenetic furrow and R8 photoreceptor cells are seen to develop in the clone. The partial rescue is not increased by increased Ras85D^D38E copy number nor by the addition of Ras85D^Y40C.

(Halfar et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ras85D^D38E

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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