FlyBase Allele Report: Dmel\Gαs[R19]

General Information

Symbol

Dmel\Gαs^R19

Species

D. melanogaster

Name

FlyBase ID

FBal0126532

Feature type

allele

Associated gene

Dmel\Gαs

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wolfgang et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: W265term.

(Wolfgang et al., 2001)

Nucleotide substitution: G795A.

(Wolfgang et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,946,069..23,946,069 [+]

Nucleotide change:

G23946069A

Reported nucleotide change:

G795A

Amino acid change:

Reported amino acid change:

W265term

(Wolfgang et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Wolfgang et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

G-sα60A^R19/+ flies have a significantly lower intake of various sugars: trehalose, sucrose, fructose or glucose. This effect is seen with various concentrations of trehalose (10-80mM), but is not seen with bitter substances: caffeine, quinine or denatonium benzoate.

(Ueno et al., 2006)

34% of G-sα60A^R19/Df(2R)or-BR11 embryos die, the remaining larvae die either as they hatch or shortly after hatching. Larvae that hatch show little or no movement and do not grow. Ovaries of females carrying homozygous germ-line clones appear normal. 11% of G-sα60A^R60/G-sα60A^R19 embryos die. 44% of G-sα60A^R60/G-sα60A^R19 embryos derived from females carrying G-sα60A^R60 homozygous germ-line clones die. G-sα60A^R79/G-sα60A^R19 embryos derived from females carrying G-sα60A^R79 homozygous germ-line clones do not have cuticle defects. G-sα60A^R60/G-sα60A^R19 embryos derived from females carrying G-sα60A^R60 or G-sα60A^R19 homozygous germ-line clones do not have cuticle defects. 97% of G-sα60A^B19/G-sα60A^R19 embryos derived from females carrying G-sα60A^B19 homozygous germ-line clones do not have cuticle defects.

(Wolfgang et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Gαs^R19/G-salpha60A[+] is rescued by Gαs^UAS.cCa/Scer\GAL4^Gr5a.PU

(Ueno et al., 2006)

Comments

Stimulation of long labellar taste bristles in G-sα60A^R19/Scer\GAL4^Tre.PU; G-sα60A^Scer\UAS.cCa flies with trehalose induces significantly more biphasic spikes in associated sensory neurons than seen in G-sα60A^R19/+ flies but not significantly more than that seen in wild-type flies.

(Ueno et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: a second site recessive lethal mutation on the same chromosome.

(Wolfgang et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DGsα^R19

(Ueno et al., 2006)

G-sα60A^R19

Gαs^R19

dgs^R19

(Wolfgang et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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