FlyBase Allele Report: Dmel\tko[25t.tTa]

General Information

Symbol

Dmel\tko^25t.tTa

Species

D. melanogaster

Name

FlyBase ID

FBal0127868

Feature type

allele

Associated gene

Dmel\tko

Associated Insertion(s)

Carried in Construct

P{tko^25t.tTa}

Also Known As

P{tko^25t}

Key Links

Transgenic product class

missense_variant

(Toivonen et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Toivonen et al., 2001)

Mutagen

in vitro construct

(Toivonen et al., 2001)

Progenitor genotype

tko^25t

(Toivonen et al., 2001)

Carried in construct

P{tko^25t.tTa}

Cytology

Description

3.2kb BamHI genomic fragment containing the mutant tko^25t coding region and flanking sequences.

(Toivonen et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

tko

Encoded product / tool

tko

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with tko³/tko^25t

(Toivonen et al., 2003)

bang sensitive, with tko³/tko^25t

(Toivonen et al., 2003)

partially lethal - majority die, with tko³/tko^25t

(Toivonen et al., 2003)

short lived, with tko³/tko^25t

(Toivonen et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

tko³/tko^25t transheterozygotes carrying additional one or three copies of the tko^25t.tTa mutant allele on a transgene improved the phenotypic defects of the transheterozygotes: the developmental delay is reduced, greater fraction of the flies manage to eclose, the adults show long but measurable recovery times in the bang-sensitivity assay and greatly reduced adult lifespan.

(Toivonen et al., 2003)

Flies carrying tko^25t.tTa in a wild-type background do not show bang sensitivity and show no developmental delay.

(Toivonen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tko^25t.tTa, tko^25t has abnormal developmental rate phenotype, suppressible by mRpL14^{GreenH-Pelican}

(Kemppainen et al., 2009)

tko^25t.tTa, tko^25t has bang sensitive phenotype, suppressible by mRpL14^{GreenH-Pelican}

(Kemppainen et al., 2009)

NOT suppressed by

Statement

Reference

tko^25t.tTa, tko³ has abnormal developmental rate phenotype, non-suppressible by Dp(1;1)weeb¹

(Kemppainen et al., 2009)

tko^25t.tTa, tko³ has bang sensitive phenotype, non-suppressible by Dp(1;1)weeb¹

(Kemppainen et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

A combination of mRpL14^{GreenH-Pelican} and tko^25t.tTa fails to rescue bang sensitivity or the delay in eclosion seen in tko^25t mutants.

Dp(1)weeb¹/tko³ mutants retain a developmental delay even when an expressed, ectopic copy of tko^25t.tTa is also present.

(Kemppainen et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

tko^25t.tTa fails to rescue tko^25t

(Kemppainen et al., 2009)

tko^25t.tTa fails to rescue tko^25t

(Toivonen et al., 2001)

tko^25t.tTa fails to rescue tko³

(Toivonen et al., 2001)

Comments

Ectopic copies of tko^25t.tTa fails to rescue bang sensitivity in tko^25t mutants and only a minimal rescue of developmental delay.

A combination of mRpL14^{GreenH-Pelican} and tko^25t.tTa fails to rescue bang sensitivity or the delay in eclosion seen in tko^25t mutants.

(Kemppainen et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tko^25t.tTa

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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