FlyBase Allele Report: Dmel\Amph[A.UAS]

General Information

Symbol

Dmel\Amph^A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0128039

Feature type

allele

Associated gene

Dmel\Amph

Associated Insertion(s)

Carried in Construct

P{UAS-Amph.A}

Also Known As

UAS-Amph

Key Links

Transgenic product class

cDNA

(Zelhof et al., 2001)

wild_type

(Zelhof et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zelhof et al., 2001)

wild_type

(Zelhof et al., 2001)

Mutagen

in vitro construct

(Razzaq et al., 2001, Zelhof et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Amph.A}

Cytology

Description

UASt regulatory sequences drive expression of the Amph A isoform (EST LD19810).

(Zelhof et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Amph

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\MAPT^4xA.UAS.2N4R

(Chapuis et al., 2013)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Yu et al., 2020)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Bernstein et al., 2016)

modeled by Hsap\MAPT^V337M.UAS

(Bernstein et al., 2016)

modeled by Hsap\MAPT^R406W.UAS

(Bernstein et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

rhabdomere, with Scer\GAL4^GMR.PF

(Zelhof et al., 2001)

rhabdomere | ectopic, with Scer\GAL4^GMR.PF

(Zelhof et al., 2001)

Detailed Description

Statement

Reference

Expression of Amph^A.Scer\UAS under the control of Scer\GAL4^GMR.PF results in split or ectopic rhabdomeres or the loss of rhabdomeres. These photoreceptor neurons are functional, despite the morphological defects.

(Zelhof et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Amph^A.UAS, Scer\GAL4^{elav.Switch.PO} is a suppressor | partially of abnormal locomotor behavior | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Yu et al., 2020)

Amph^A.UAS, Scer\GAL4^{elav.Switch.PO} is a suppressor | partially of short lived | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Yu et al., 2020)

Phenotype Manifest In

Suppressor of

Statement

Reference

Amph^A.UAS, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\MAPT^4xA.UAS.2N4R, Scer\GAL4^GMR.PF

(Chapuis et al., 2013)

NOT Suppressor of

Statement

Reference

Amph^A.UAS, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\MAPT^4xA.UAS.2N4R, Scer\GAL4^GMR.PF

(Chapuis et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Eye-specific overexpression of Amph^A.Scer\UAS (under the control of Scer\GAL4^GMR.PF) does not significantly alter the size of Hsap\MAPT^{4xA.Scer\UAS.2N4R}-induced rough eye, although the number of ommatidia is significantly reduced due to increased ommatidial fusions.

(Chapuis et al., 2013)

Complementation and Rescue Data

Partially rescues

Amph^A.UAS/Scer\GAL4^hs.PB partially rescues Amph²⁶

(Razzaq et al., 2001)

Comments

Most Amph²⁶ flies rescued with Amph^A.Scer\UAS (driven by Scer\GAL4^hs.PB) can fly and exhibit varying degrees of recovery of their T-system.

(Razzaq et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (3)