FB2025_05 , released December 11, 2025
Gene: Dmel\Amph
Open Close
General Information
Symbol
Dmel\Amph
Species
D. melanogaster
Name
Amphiphysin
Annotation Symbol
CG8604
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027356
Gene Model Status
Current
Stock Availability
22 publicly available
Gene Summary
Amphiphysin (Amph) encodes a BAR and SH3 domain containing protein that regulates muscle transverse tubule formation. It exhibits post-synaptic localization at the neuromuscular junction and its mutants show locomotion defects. [Date last reviewed: 2018-09-27] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Damp, damph

Key Links
Genomic Location
Cytogenetic map
49B7-49B8
Sequence location
Recombination map
2-67
RefSeq locus
NT_033778 REGION:12593604..12612068
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables phospholipid binding
inferred from biological aspect of ancestor with PANTHER:PTN002509858
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P49418
(Gene Ontology Curators, 2002-)
Biological Process (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in intracellular protein localization
inferred from mutant phenotype
(Zelhof et al., 2001)
involved_in nephrocyte diaphragm assembly
inferred from genetic interaction with FLYBASE:scramb1; FB:FBgn0052056
(Castillo-Mancho et al., 2024)
involved_in regulation of muscle contraction
inferred from mutant phenotype
(Razzaq et al., 2001)
involved_in rhabdomere development
inferred from mutant phenotype
(Zelhof et al., 2001)
involved_in rhabdomere membrane biogenesis
inferred from mutant phenotype
(Zelhof et al., 2001)
NOT involved_in synaptic vesicle endocytosis
inferred from mutant phenotype
(Leventis et al., 2001, Razzaq et al., 2001, Zelhof et al., 2001)
inferred from expression pattern
(Razzaq et al., 2001)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in exocytosis
traceable author statement
(Packard et al., 2003)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cell cortex
inferred from direct assay
(Castillo-Mancho et al., 2024)
located_in cleavage furrow
inferred from direct assay
(Yan et al., 2013)
located_in plasma membrane
inferred from direct assay
(Zelhof et al., 2001)
located_in synapse
inferred from direct assay
(Leventis et al., 2001, Zelhof et al., 2001)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from electronic annotation with InterPro:IPR004148
(InterPro Project Members, 2004-)
is_active_in plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN002509858
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Amphiphysin (Amph) encodes a BAR and SH3 domain containing protein that regulates muscle transverse tubule formation. It exhibits post-synaptic localization at the neuromuscular junction and its mutants show locomotion defects. [Date last reviewed: 2018-09-27]
Summary (Interactive Fly)

SH3 domain protein that functions in membrane morphogenesis - involved in cellularization and plays a role in establishment of proper localization of postsynaptic proteins

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Amph for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7KLE5)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Amph-RA
FBtr0087828
NM_078993
3184
602
Amph-RB
FBtr0333099
NM_001273990
2884
502
Amph-RC
FBtr0333100
NM_001273991
2497
373
Additional Transcript Data and Comments
Reported size (kB)

3.162 (longest cDNA)

(Razzaq et al., 2000)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Amph-PA
FBpp0086941
65.9
602
4.89
Q7KLE5
NP_523717
AAF58486
Amph-PB
FBpp0305312
55.2
502
5.32
A0A0B4KEW6
NP_001260919
AGB93452
Amph-PC
FBpp0305313
42.3
373
6.20
A0A0B4KFR7
NP_001260920
AGB93453
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

602 (aa); 66 (kD predicted)

(Razzaq et al., 2000)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Amph using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.19

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 3 -- 4
furrow canal
(Zelhof et al., 2001)
furrow canal
(Zelhof et al., 2001)
embryonic stage 4 -- 5
organism
(Leventis et al., 2001)
embryonic stage 5
somatic cell
(Zelhof et al., 2001)
embryonic stage 9
ventral ectoderm
(Zelhof et al., 2001)
neuroblast
(Zelhof et al., 2001)
embryonic stage 10 -- 17
epithelium
(Leventis et al., 2001)
embryonic foregut
(Leventis et al., 2001)
embryonic hindgut
(Leventis et al., 2001)
embryonic stage 15
embryonic/larval tracheal section
(Zelhof et al., 2001)
embryonic esophagus
(Zelhof et al., 2001)
embryonic garland cell
(Zelhof et al., 2001)
embryonic stage 15 -- 16
presumptive embryonic salivary gland
(Zelhof et al., 2001)
presumptive embryonic salivary gland
(Zelhof et al., 2001)
embryonic stage 16
embryonic epidermis
(Zelhof et al., 2001)
embryonic hindgut
(Zelhof et al., 2001)
embryonic hindgut
(Zelhof et al., 2001)
third instar larval stage
epithelial cell
(Leventis et al., 2001)
larval somatic muscle cell
(Leventis et al., 2001)
embryonic/larval neuromuscular junction
(Leventis et al., 2001, Zelhof et al., 2001)
pupal stage P6
photoreceptor neuron

Comment: 24 hr APF; localized throughout cell

(Zelhof et al., 2001)
pharate adult stage P8
photoreceptor neuron

Comment: 48 hr APF; localized where rhabdomeres will develop

(Zelhof et al., 2001)
adult stage
cone cell
(Zelhof et al., 2001)
adult head
(Zelhof et al., 2001)
oogenesis stage S7 -- S9 & adult stage | female
border follicle cell

Comment: trailing

(Szafranski and Goode, 2004)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
organism
(Leventis et al., 2001)
third instar larval stage
organism
(Leventis et al., 2001)
larval somatic muscle cell & third instar larval cuticle

Comment: 45, 80 and 85 kD isoforms

(Leventis et al., 2001)
larval central nervous system

Comment: 80 and 85 kD isoforms only

(Leventis et al., 2001)
imaginal disc

Comment: 80 and 85 kD isoforms only

(Leventis et al., 2001)
Additional Descriptive Data

Amph protein is distributed in a polarized fashion in clusters of migrating border follicle cells, being present at high levels in the trailing border cells.

(Szafranski and Goode, 2004)

Amph protein is first detected in cellularizing embryos at the sites of developing cell membranes. Later, Amph is localized to epithelial tissues and the embryonic foregut and hindgut. In third instar larvae, Amph protein is cytoplasmic, and found in epithelial cells and somatic muscles. In the later, Amph is localized to the postsynaptic membrane of neuromuscular junctions.

(Leventis et al., 2001)

Amph colocalizes with postsynaptic marker dlg1, but not presynaptic marker Csp, at the embryonic/larval neuromuscular junction

(Zelhof et al., 2001)
Marker for
 
border follicle cell
(Szafranski and Goode, 2004)
Subcellular Localization
CV Term
Evidence
References
located_in cell cortex
inferred from direct assay
(Castillo-Mancho et al., 2024)
located_in cleavage furrow
inferred from direct assay
(Yan et al., 2013)
located_in plasma membrane
inferred from direct assay
(Zelhof et al., 2001)
located_in synapse
inferred from direct assay
(Leventis et al., 2001, Zelhof et al., 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Amph in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 7-8
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 23 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Amph
Transgenic constructs containing regulatory region of Amph
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene disrupted in
Df(2R)Amph26
(Razzaq et al., 2001)
Df(2R)Exel7121
(Ryder, 2004.4.26)
Df(2R)vg-C
(Razzaq et al., 2001)
Inferred from location ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal locomotor behavior | adult stage
abnormal locomotor behavior | adult stage (with Amph5E3)
abnormal locomotor behavior | adult stage (with AmphMI08903-TG4.0)
abnormal locomotor behavior | third instar larval stage
abnormal neuroanatomy | larval stage, with Scer\GAL4elav-C155
abnormal neuroanatomy | third instar larval stage
abnormal neurophysiology
abnormal neurophysiology | larval stage
decreased rate of movement
decreased rate of movement | adult stage
decreased rate of movement | larval stage
flightless
Phenotype manifest in
Allele
abdominal 1 lateral transverse muscle cell | embryonic stage
abdominal 1 lateral transverse muscle cell | embryonic stage, with Scer\GAL4twi.PU
anterior fascicle & synapse, with Scer\GAL4elav-C155
indirect flight muscle & myofibril
indirect flight muscle cell
larval somatic muscle cell | third instar larval stage
macrochaeta
motor neuron
muscle cell of ventral longitudinal muscle 3 | larval stage
muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4Mef2.PU
muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4Mhc.PU
muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4twi.PU
nephrocyte
neuromuscular junction | larval stage
NMJ bouton | larval stage
nucleus
nucleus, with Scer\GAL4Mef2.PU
nucleus, with Scer\GAL4Mhc.PU
nucleus, with Scer\GAL4twi.PU
nucleus | third instar larval stage
rhabdomere
rhabdomere, with Scer\GAL4GMR.PF
rhabdomere | ectopic, with Scer\GAL4GMR.PF
rhabdomere of eye photoreceptor cell | adult stage, with Scer\GAL4GMR.PU
rhabdomere of eye photoreceptor cell | P-stage, with Scer\GAL4GMR.PU
slit diaphragm
T-tubule
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (10)
Hsap\AMPH
12 of 14
Yes
Yes
3  
Hsap\BIN1
11 of 14
No
Yes
 
6  
Hsap\BIN2
9 of 14
No
Yes
Hsap\BIN3
2 of 14
No
Yes
Hsap\ARHGEF37
1 of 14
No
No
Hsap\ARHGEF38
1 of 14
No
No
Hsap\DNMBP
1 of 14
No
No
Hsap\ITSN1
1 of 14
No
No
Hsap\MYO1F
1 of 14
No
No
Hsap\PACSIN2
1 of 14
No
No
1  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (10)
Rnor\Amph
12 of 14
Yes
Yes
Rnor\Bin1
11 of 14
No
Yes
Rnor\Bin2
10 of 14
No
Yes
Rnor\Bin3
2 of 14
No
Yes
Rnor\Arhgap10
1 of 14
No
No
Rnor\Arhgef37
1 of 14
No
No
Rnor\Dnmbp
1 of 14
No
No
Rnor\Itsn1
1 of 14
No
No
Rnor\Myo1f
1 of 14
No
No
Rnor\Pacsin2
1 of 14
No
No
Mus musculus (laboratory mouse) (8)
Mmus\Amph
11 of 14
Yes
Yes
Mmus\Bin1
11 of 14
Yes
Yes
Mmus\Bin2
10 of 14
No
Yes
Mmus\Bin3
2 of 14
No
Yes
Mmus\Arhgef37
1 of 14
No
No
Mmus\Arhgef38
1 of 14
No
No
Mmus\Dnmbp
1 of 14
No
Yes
Mmus\Zan
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (7)
Xtro\bin2
8 of 13
Yes
Yes
Xtro\amph
6 of 13
No
Yes
Xtro\bin1
6 of 13
No
Yes
Xtro\hesx1
1 of 13
No
Yes
Xtro\myo1e.1
1 of 13
No
No
Xtro\myo1f
1 of 13
No
No
Xtro\pacsin2
1 of 13
No
No
Danio rerio (Zebrafish) (13)
Drer\amph
12 of 14
Yes
Yes
Drer\bin1b
12 of 14
Yes
Yes
Drer\bin2b
11 of 14
No
Yes
Drer\bin2a
8 of 14
No
Yes
Drer\arhgef38
1 of 14
No
No
Drer\bin3
1 of 14
No
Yes
Drer\dbnlb
1 of 14
No
No
Drer\dnmbp
1 of 14
No
No
Drer\itsn2b
1 of 14
No
No
Drer\LOC563561
1 of 14
No
Yes
Drer\myo1eb
1 of 14
No
No
Drer\sh3gl2b
1 of 14
No
No
Drer\sh3gl3b
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\amph-1
10 of 14
Yes
Yes
Cele\Y37A1B.17
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP005076
11 of 12
Yes
Yes
Agam\AgaP_AGAP004625
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\RVS161
5 of 13
Yes
Yes
Scer\RVS167
5 of 13
Yes
Yes
Scer\BOI1
1 of 13
No
No
Scer\BOI2
1 of 13
No
No
Scer\LSB1
1 of 13
No
No
Scer\PIN3
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\hob3
5 of 12
Yes
Yes
Spom\hob1
4 of 12
No
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Amph. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
Synd
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
BIN1; bridging integrator 1
model of  centronuclear myopathy 2
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 7 )
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
AMPH; amphiphysin
12 of 14
600418
       
      BIN1; bridging integrator 1
      11 of 14
      BRIDGING INTEGRATOR 1; BIN1
       
      BIN2; bridging integrator 2
      9 of 14
      605936
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Dmel gene
          Ortholog showing functional complementation
          Supporting References
          Amph
          Hsap\BIN1
          (Lambert et al., 2022)
          Interactions
          Summary of Physical Interactions
          Summary of Genetic Interactions
          Interaction Browsers
          View in FlyBase Interactions Browser   View in MIST tool (external link)
          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Amph
          enhanceable
          Fas2
          (Mathew et al., 2003)
          Amph
          enhanceable
          scramb1
          (Castillo-Mancho et al., 2024)
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          bif
          suppressible
          Amph
          (Zelhof et al., 2001)
          Fas2
          enhanceable
          Amph
          (Mathew et al., 2003)
          Past1
          suppressible
          Amph
          (Koles et al., 2015)
          scramb1
          enhanceable
          Amph
          (Castillo-Mancho et al., 2024)
          External Data
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          FlyBase
          External Links
          External Data
          Linkouts
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          SignaLink - A signaling pathway resource with multi-layered regulatory networks.
          Class of Gene
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          2R
          Recombination map
          2-67
          Cytogenetic map
          49B7-49B8
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          49B7-49B8
          Limits computationally determined from genome sequence between P{EP}CG8569EP1182 and P{EP}AmphEP2175&P{lacW}spt4k05316
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          49B-49B
          (Lloyd et al., 2000, Razzaq, 1999.7.23)
          49B-49B
          (determined by in situ hybridisation)
          (Razzaq et al., 2000)
          46B6-46B11
          (Zhang, 1999.6.11)
          Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.
          (Lloyd et al., 2000)
          Experimentally Determined Recombination Data
          Location

          2-67

          (FlyBase, 2016)

          2-69.0

          (Comeron et al., 2012)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (22)
          Genomic Clones (22)
          cDNA Clones (69)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Szafranski and Goode, 2004, Leventis et al., 2001, Zelhof et al., 2001)
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                The structure of the Amph BAR domain has been solved: It is a crescent-shaped dimer that binds preferentially to highly curved negatively charged membranes.

                (Peter et al., 2004)

                dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                (Kiger et al., 2003)

                Amph functions during SNARE-dependent postsynaptic Fas2 membrane cycling.

                (Mathew et al., 2003)

                Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

                (Claridge-Chang et al., 2001)

                Area matching Drosophila EST AA540348.

                (Davies, 2001.3.15)

                Amph is involved in the structural organisation of the membrane-bound compartments of the excitation-contraction coupling machinery of muscles, but not in synaptic vesicle endocytosis.

                (Razzaq et al., 2001)

                Amph is localised to actin-rich membrane domains in many cell types. Overall orientation not stated: Sin3A- Amph+ Gα49B+

                (Zelhof et al., 2001)
                Relationship to Other Genes
                Source for database merge of
                Additional comments
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (15)
                Reported As
                Symbol Synonym
                AMPH
                (Park et al., 2023)
                Amp
                Amph
                (Warecki et al., 2025, Miao et al., 2024, Biton et al., 2023, Titlow et al., 2023, Evans et al., 2021, Giong et al., 2021, Kapoor et al., 2021, Okada et al., 2021, Pang et al., 2021, Slaidina et al., 2021, Wei et al., 2021, Azevedo and Baylies, 2020, Yu et al., 2020, Sivanantharajah et al., 2019, Dourlen et al., 2018, Collins et al., 2017, Dourlen et al., 2017, Transgenic RNAi Project members, 2017-, Bernstein et al., 2016, Gene Disruption Project members, 2016-, Safi et al., 2016, Lee et al., 2015, Peterson and Krasnow, 2015, Van Bortle et al., 2015, Acharya et al., 2014, Luck et al., 2014, Merlo et al., 2014, Chapuis et al., 2013, Esslinger et al., 2013, Lee and Harris, 2013, Yan et al., 2013, Zhu and Zhang, 2013, Felix et al., 2012, Hazelett et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Paik et al., 2012, Jung et al., 2010, Sokac and Wieschaus, 2008, Stuart et al., 2007, Wei et al., 2004)
                Bin1
                (Dräger et al., 2017)
                CG8604
                (National Institute of Genetics Fly Stocks, 2022-, Trivedi et al., 2020, Cammarato et al., 2011, Khanna et al., 2010, Ni et al., 2010.12.1, Perkins et al., 2009, Norga et al., 2003, Kraut et al., 2001, Kraut et al., 2001, Verstreken and Bellen, 2000.9.4)
                DAMP
                (Lloyd et al., 2000)
                Damp
                (Chow et al., 2001, Leventis et al., 2000)
                amph
                (Sauerwald et al., 2019, Reuveny et al., 2018, Koles et al., 2015, Su et al., 2013, Elliott and Sparrow, 2012, Ribeiro et al., 2011, Rocha et al., 2011, Quinones et al., 2010, Korolchuk et al., 2007, Deshpande et al., 2004, Chow et al., 2003, Rogat and Miller, 2002, Chow et al., 2001, Leventis et al., 2001, Razzaq et al., 2001, Zelhof et al., 2001)
                dAmp
                (Yoon et al., 2012)
                dAmph
                (Peter et al., 2004)
                damph
                (Mathew et al., 2003, Packard et al., 2003)
                Name Synonyms
                Amphiphysin
                (Azevedo and Baylies, 2020, Schmidt and Grosshans, 2018, Balakrishnan et al., 2015, Lee et al., 2015, Lee and Harris, 2013, Zheng et al., 2013, Cammarato et al., 2011, Khanna et al., 2010, Sokac and Wieschaus, 2008, Jordan et al., 2007, Cirelli et al., 2005, Habermann, 2004, Wei et al., 2004, Zelhof et al., 2003, Razzaq et al., 2000)
                amphiphysin
                (Okada et al., 2021, Latcheva et al., 2019, Hutchison et al., 2016, Isas et al., 2015, Merlo et al., 2014, Su et al., 2013, Elliott and Sparrow, 2012, Yoon et al., 2012, Rocha et al., 2011, Jung et al., 2010, Quinones et al., 2010, Arkhipov et al., 2009, Arkhipov et al., 2008, Williams et al., 2004, Claridge-Chang et al., 2001, Bellen, 2000.12.25, Prokopenko et al., 2000)
                amphiphysin/BIN1
                (Adam et al., 2015)
                dAmphiphysin
                (Habermann, 2004)
                Secondary FlyBase IDs
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 64 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  FlyMine - An integrated database for Drosophila genomics
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  PDB - An information portal to biological macromolecular structures
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                  DroID - A comprehensive database of gene and protein interactions.
                  DRSC - Results frm RNAi screens
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Flygut - An atlas of the Drosophila adult midgut
                  FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                  KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                  SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                  References (152)