FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Amph5E3
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General Information
Symbol
Dmel\Amph5E3
Species
D. melanogaster
Name
FlyBase ID
FBal0137668
Feature type
allele
Associated gene
Dmel\Amph
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Leventis et al., 2001)
Mutagen
P-element activity
(Leventis et al., 2001)
Progenitor genotype
AmphEP2175
(Leventis et al., 2001)
Cytology
Description

Imprecise excision of the P{EP} element, resulting in deletion of the entire Amph gene.

(Leventis et al., 2001)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Alzheimer's disease
      modeled by Hsap\MAPT4xA.UAS.2N4R
      (Chapuis et al., 2013)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The kinetics of cleavage furrow ingression during cellularisation are altered in mutant embryos compared to wild type. All three phases of ingression are faster than normal, with the final phase in particular being significantly faster than wild type.

      (Su et al., 2013)

      Mutant larvae and adults are sluggish. Third instar larvae show about a 70% reduction in movement compared to controls. The number of boutons and amount of branching at the neuromuscular junction appear normal in mutant third instar larvae. The amplitude of miniature excitatory junctional currents at the neuromuscular junction in mutant third instar larvae is not significantly different from that of control larvae. Recovery from synaptic fatigue is not altered in mutant larvae compared to controls. There is no major difference in CNS-generated motor activity in mutant and control larvae.

      (Leventis et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Amph[+]/Amph5E3 is a suppressor of notal microchaeta phenotype of Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4Eq

      (Chapuis et al., 2013)

      Amph[+]/Amph5E3 is a suppressor of eye phenotype of Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF

      (Chapuis et al., 2013)

      Amph[+]/Amph5E3 is a suppressor of ommatidium phenotype of Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF

      (Chapuis et al., 2013)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      A Amph5E3 heterozygous background suppresses Hsap\MAPT4xA.Scer\UAS.2N4R-induced toxicity, leading to a 30% increase in eye surface, compared with flies expressing solely Hsap\MAPT4xA.Scer\UAS.2N4R.

      A Amph5E3 heterozygous background suppresses the Scer\GAL4Eq1>Hsap\MAPT4xA.Scer\UAS.2N4R-induced a bristle loss phenotype, resulting in 37% more notal bristles compared with flies expressing Scer\GAL4Eq1>Hsap\MAPT4xA.Scer\UAS.2N4R alone.

      A Amph5E3 mutant background does not affect the subtle rough eye phenotype caused by expression of Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4elav-C155.

      (Chapuis et al., 2013)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Amph5E3
      (Lambert et al., 2022, Chapuis et al., 2013)
      amph5E3
      (Sheng et al., 2018, Su et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)