FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\crbextraTM.UAS.GFP
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General Information
Symbol
Dmel\crbextraTM.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0134114
Feature type
allele
Associated gene
Dmel\crb
Associated Insertion(s)
Carried in Construct
P{UAS-crb.extra.TM-GFP}
Also Known As
UAS-crbextraTM-GFP
Key Links
Transgenic product class
inframe_deletion
(Pellikka et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Pellikka et al., 2002)
Mutagen
in vitro construct
(Pellikka et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-crb.extra.TM-GFP}
Cytology
Description

Expression of a C-terminal truncated (by 31 amino acids) crb protein, tagged with GFP, is governed by UASt regulatory sequences.

(Pellikka et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
crb
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of crbextraTM.UAS.GFP under the control of Scer\GAL4ninaE.PTa induces rhabdomere loss in the ommatidium and large cyst-like expansions of the interrhabdomeral space, suggesting a dramatic overgrowth of the stalk membrane.

      crb11A22 mutants expressing crbextraTM.UAS.GFP under the control of Scer\GAL4GMR.PU show ommatidium defects, with a 'fused rhabdomere' phenotype; one week of continuous light exposure (light stress) induces more severe photoreceptor cell defects, including occasional cell death, as compared to wild-type controls.

      (Pellikka and Tepass, 2017)

      Expression of crbextraTM.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4nub.PU results in a significant reduction in adult wing size, and loss of crossveins in the wing, as compared to controls.

      (Nguyen et al., 2016)

      Expression of crbextraTM.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ey.PH fully rescues the crb11A22 head overgrowth phenotype.

      (Richardson and Pichaud, 2010)

      Over-expression of crbextraTM.Scer\UAS.T:Avic\GFP but not crbextra.Scer\UAS or crbintra.Scer\UAS causes expansion of the photoreceptor cell stalk membrane similar to that seen with crbScer\UAS.cWa.

      (Pellikka et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4nub.PU, crbextraTM.UAS.GFP has wing phenotype, non-suppressible by ebiΔWD7-8.UAS/Scer\GAL4nub.PU

      (Nguyen et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of ebiScer\UAS.Exel, but not ebiΔWD7-8.Scer\UAS, partially suppresses the reduced wing size seen in flies expressing crbextraTM.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4nub.PU.

      (Nguyen et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      crbextraTM.UAS.GFP/Scer\GAL4ey.PH rescues crb11A22

      (Richardson and Pichaud, 2010)
      Fails to rescue

      crbextraTM.UAS.GFP/Scer\GAL4GMR.PU fails to rescue crb11A22

      (Pellikka and Tepass, 2017)

      crbextraTM.UAS.GFP/Scer\GAL4nullo.PG fails to rescue crb11A22

      (Letizia et al., 2013)
      Comments

      Expression of crbextraTM.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4nullo.PG fails to rescue the defects in cell shape which is seen in the dorsal cells of crb11A22 embryos.

      (Letizia et al., 2013)

      Expression of crbextraTM.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ey.PH fully rescues the crb11A22 head overgrowth phenotype.

      (Richardson and Pichaud, 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Crumbs-extra-TM
      (Herranz et al., 2006)
      crbECD
      (Hafezi et al., 2012)
      crbextraTM.Scer\UAS.T:Avic\GFP
      crbextraTM.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (10)