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General Information
Symbol
Dmel\Rac1Y40C.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0135832
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutated form of Rac1 (carries the amino acid replacement Y40C) tagged at the N-terminal end with two copies of Tag:MYC.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Rac1Y40C.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4OK107 does not disrupt mushroom body axon patterning in a wild-type background.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Rac1Y40C.Scer\UAS.T:Hsap\MYC suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107. This suppression does not occur in a sickΔ/sickA384 mutant background.

Mushroom body axon growth defects in single-cell Rac1J11 Rac2Δ MtlΔ γ neuron clones are largely rescued by expression of Rac1Y40C.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4OK107. 81% of axons in Rac1J11 Rac2Δ mushroom body clones show mutant phenotypes, predominantly guidance (55%) and branching (24%) defects. Expression of Rac1Y40C.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4OK107 does not reduce the total percentage of axonal defects, but results in a marked shift in the distribution of defects, with most showing branching (45%) rather than guidance (31%) defects.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Rac1Y40C.Scer\UAS.T:Hsap\MYC
Rac1Y40C.UAS.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (3)