FlyBase Allele Report: Dmel\dsf[VP16.UAS]

General Information

Symbol

Dmel\dsf^VP16.UAS

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Pitman

FlyBase ID

FBal0137275

Feature type

allele

Associated gene

Dmel\dsf

Associated Insertion(s)

Carried in Construct

P{UAS-dsf.VP16}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pitman et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-dsf.VP16}

Cytology

Description

UASt regulatory sequences drive expression of the VP16 activation domain fused to full-length dsf.

(Pitman et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile, with Scer\GAL4^dpp.blk1

(Pitman et al., 2002)

fertile, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

lethal | embryonic stage, with Scer\GAL4^elav.PLu

(Pitman et al., 2002)

lethal | larval stage, with Scer\GAL4^elav.PLu

(Pitman et al., 2002)

lethal - all die before end of larval stage, with Scer\GAL4^h-1J3

(Pitman et al., 2002)

lethal - all die before end of P-stage, with Scer\GAL4^Alk.6.5

(Pitman et al., 2002)

lethal - all die during embryonic stage, with Scer\GAL4^Act5C.PI

(Pitman et al., 2002)

lethal - all die during embryonic stage, with Scer\GAL4^Dll-md23

(Pitman et al., 2002)

some die during embryonic stage, with Scer\GAL4^Act5C.PI

(Pitman et al., 2002)

some die during embryonic stage, with Scer\GAL4^Dll-md23

(Pitman et al., 2002)

some die during larval stage, with Scer\GAL4^h-1J3

(Pitman et al., 2002)

some die during pharate adult stage, with Scer\GAL4^Alk.6.5

(Pitman et al., 2002)

viable, with Scer\GAL4^dpp.blk1

(Pitman et al., 2002)

viable, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

wild-type, with Scer\GAL4^dpp.blk1

(Pitman et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

metathoracic leg, with Scer\GAL4^Alk.6.5

(Pitman et al., 2002)

ommatidium, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

pigment cell, with Scer\GAL4^GMR.PF

(Pitman et al., 2002)

Detailed Description

Statement

Reference

When dsf^{Scer\UAS.T:Hsim\VP16} is driven by Scer\GAL4^Act5C.PI or Scer\GAL4^Dll-md23 an embryonic lethal phenotype is seen. When driven by Scer\GAL4^elav.PLu animals die as embryos or first instar larvae. When driven by Scer\GAL4^h-1J3 animals die as first instar larvae. When dsf^{Scer\UAS.T:Hsim\VP16} is driven by Scer\GAL4^Alk.6.5 animals die as pharate adults with crooked hind legs and foreshortened middle legs. When dsf^{Scer\UAS.T:Hsim\VP16} is driven by Scer\GAL4^dpp.blk1 no defects are detectable. When driven by Scer\GAL4^GMR.PF adults are viable and fertile, with moderate ommatidial disruption, bristle and pigment loss.

(Pitman et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dsf^{Scer\UAS.T:Hsim\VP16}

dsf^VP16.UAS

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Pitman

(Pitman et al., 2002)

Secondary FlyBase IDs

References (1)

Report Sections

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