FlyBase Allele Report: Dmel\Fmr1[FS]

General Information

Symbol

Dmel\Fmr1^FS

Species

D. melanogaster

Name

FlyBase ID

FBal0137499

Feature type

allele

Associated gene

Dmel\Fmr1

Associated Insertion(s)

Carried in Construct

P{Fmr1.FS}

Also Known As

Key Links

Transgenic product class

frameshift_variant

(Dockendorff et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

frameshift_variant

(Dockendorff et al., 2002)

Mutagen

in vitro construct

(Dockendorff et al., 2002)

Progenitor genotype

Fmr1^+t14

(Dockendorff et al., 2002)

Carried in construct

P{Fmr1.FS}

Cytology

Description

Frameshift mutation has been introduced at NcoI site in coding exon 8 of Fmr1.

(Dockendorff et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

Fmr1

Encoded product / tool

Fmr1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of fragile X syndrome

CEA with Fmr1³

(Choi et al., 2010)

CEA

(Choi et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of fragile X syndrome

is ameliorated by Pde4^dnc-1

(Choi et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior, with Fmr1³

(Choi et al., 2010)

abnormal memory, with Fmr1³

(Choi et al., 2010)

abnormal memory | adult stage

(Choi et al., 2015)

abnormal neuroanatomy | adult stage

(Choi et al., 2015)

Phenotype Manifest In

adult mushroom body

(Choi et al., 2015)

Detailed Description

Statement

Reference

Fmr1^FS mutant adult males do not display any immediate recall memory (assayed immediately after training) or short-term memory (60 min after training) in the courtship behavior experimental paradigm (the male is paired with an unreceptive female during training and than paired with either receptive virgin or unreceptive pre-mated females for the memory experiments), their long-term memory, however, is intact. The flies display a range of cross-over defects in the brain mushroom body compared to controls.

(Choi et al., 2015)

Naive courtship activity is lower in Fmr1^FS+Fmr1³ flies than in wild-type flies (Fmr1^+t14+Fmr1³). However, this difference is less pronounced in aged adults compared to young adults.

Fmr1^FS+Fmr1³ 20-dy old flies do not show a decrease in time spent courting during the training period, indicating that no learning during training is occurring. This is in contrast to the normal learning during training observed with young flies.

The majority of Fmr1^FS+Fmr1³ aged brains do not show any detectable cell death.

(Choi et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Fmr1^FS has abnormal memory | adult stage phenotype, suppressible by Pde4^dnc-1

(Choi et al., 2015)

Suppressor of

Statement

Reference

Fmr1^FS is a suppressor of abnormal memory | adult stage phenotype of Pde4^dnc-1

(Choi et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The short-term memory defects (assayed in the courtship behavior experimental paradigm) observed in either Fmr1^FS or dnc¹ single mutant adult males are restored in the double mutants.

(Choi et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Fmr1^FS fails to rescue Fmr1³

(Kanellopoulos et al., 2012)

Fmr1^FS fails to rescue Fmr1^Δ83M/Fmr1³

(Tauber et al., 2011)

Fmr1^FS fails to rescue Fmr1³

(McBride et al., 2005)

Fmr1^FS fails to rescue Fmr1³

(Dockendorff et al., 2002)

Comments

Expression of Fmr1^FS fails to rescue the learning and memory phenotype of Fmr1³ mutants.

(Kanellopoulos et al., 2012)

Fmr1^+t14 (but not Fmr1^FS) rescues locomotor defects and excessive grooming behavior at all ages (day 5, 15, 25, 35) in Fmr1³/Fmr1^Δ83M flies; courtship behavior defects at day 15 are also rescued by Fmr1^+t14 but not Fmr1^FS.

(Tauber et al., 2011)

The defective immediate recall and short-term memory as well as frequent defects in adult mushroom body morphology characteristic for Fmr1³ mutant males are not restored by combination with a single copy of the Fmr1^FS transgene.

(McBride et al., 2005)

Fails to rescue rhythmicity and courtship defects of Fmr1³.

(Dockendorff et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Fmr1^FS

(Choi et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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