FlyBase Allele Report: Dmel\Syx5[EP2313]

General Information

Symbol

Dmel\Syx5^EP2313

Species

D. melanogaster

Name

FlyBase ID

FBal0141353

Feature type

allele

Associated gene

Dmel\Syx5

Associated Insertion(s)

P{EP}Syx5^EP2313

Carried in Construct

Also Known As

EP(2)2313

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Xu et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{EP}Syx5^EP2313

Cytology

Description

The P{EP} insertion is in the 5' regulatory region of Syx5, 803 bp upstream of the ATG, and in the correct orientation for expression of Syx5.

(Xu et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,307,337..16,307,337 [+]

(Hiramatsu et al., 2019, Maybeck and Röper, 2009, Xu et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis (with Syx5^AR113)

(Xu et al., 2002)

male sterile (with Syx5^AR113)

(Xu et al., 2002)

partially lethal - majority die (with Syx5^AR113)

(Xu et al., 2002)

wild-type, with Scer\GAL4^da.G32

(Xu et al., 2002)

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Maybeck and Röper, 2009)

endoplasmic reticulum | adult stage

(Zhao et al., 2015)

lipid droplet | adult stage

(Zhao et al., 2015)

Nebenkern (with Syx5^AR113)

(Xu et al., 2002)

photoreceptor neuron

(Zhao et al., 2015)

rhabdomere

(Zhao et al., 2015)

spermatozoon (with Syx5^AR113)

(Xu et al., 2002)

testis (with Syx5^AR113)

(Xu et al., 2002)

Detailed Description

Statement

Reference

Syx5^EP2313 homozygotes exhibit small and irregularly shaped rhabdomeres, and the photoreceptor cells show an accumulation of ER and lipid droplets, as compared to controls.

(Zhao et al., 2015)

Expression via Scer\GAL4^fkh.PH results in a weakly penetrant 'degenerating glands' phenotype in the embryonic salivary gland.

(Maybeck and Röper, 2009)

Syx5^EP2313/Syx5^AR113 mutant males (with no Scer\GAL4 driver) have no motile sperm and appear to be defective in spermatid elongation. Mutant testes contain predominantly large, oval cysts and a few elongated bundles. Mutant testes also have abnormally large mitochondrial derivatives that are associated with multiple nuclei, indicative of a failure in cytokinesis. Mutant testes also exhibit an accumulation of unusual ovoid spermatid cysts.

(Xu et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Syx5^EP2313/Syx5^AE48 is partially rescued by Scer\GAL4^da.G32, Syx5^EP2313

(Xu et al., 2002)

Partially rescues

Scer\GAL4^da.G32, Syx5^EP2313 partially rescues Syx5^EP2313/Syx5^AE48

(Xu et al., 2002)

Comments

The addition of Scer\GAL4^da.G32 to Syx5^EP2313/Syx5^AR113 animals rescues the semilethality, mostly rescues the cytokinesis phenotype and the bundle elongation defects, but no motile sperm are seen, and males remain sterile.

(Xu et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

17041

w¹¹¹⁸; P{EP}Syx5^EP2313/CyO

Kyoto

114446

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{EP}Syx5^EP2313 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

Syx5^EP2313 is homozygous lethal, but this lethality is likely to be due to second site mutations or the effect of the P{EP} element on adjacent genes, as it is only semilethal in combination with Syx5^AR113. Driving Syx5^EP2313 with Scer\GAL4^da.G32 cannot rescue the lethality, also suggesting second site mutations.

(Xu et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EP(2)2313

(Xu et al., 2002)

Syx5 EP2313P

(Zhao et al., 2015)

Syx5^EP2313

(Hiramatsu et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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