A heat shock promoter drives expression of a 0.6kb cDNA fragment encoding Su(var)3-7 amino acids 971 to 1169. The Su(var)3-7 protein is tagged at the N-terminal end with Tag:HA.
Expression of Su(var)3-7B-Ct.hs.T:Ivir\HA1 has no effect on the variegation of w (carried on the P{Winkelried}D insertion) in In(2)Heidi-P{Winkelried}D flies.