FlyBase Allele Report: Dmel\Drep1[P]

General Information

Symbol

Dmel\Drep1^P

Species

D. melanogaster

Name

FlyBase ID

FBal0141563

Feature type

allele

Associated gene

Dmel\Drep1

Associated Insertion(s)

P{lacW}Drep1^P

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Mukae et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{lacW}Drep1^P

Cytology

Description

P-element local hopping, resulting in a P{lacW} insertion in the 5'UTR of Rep1, 17bp upstream of the ATG initiation codon.

(Mukae et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death

(Mukae et al., 2002)

abnormal cell death | conditional

(Bass et al., 2009)

abnormal cell death | oogenesis stage S14

(Bass et al., 2009)

fertile

(Mukae et al., 2002)

viable

(Mukae et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Drep-1^P homozygous egg chambers from animals subjected to starvation conditions to induce mid-oogenesis programmed cell death display abnormal chromatin compaction, and nurse cell nuclei persist until late stages of degeneration. An elongated egg chamber morphology is observed. Eventually most follicle cells degenerate, and small sacs of nurse cell nuclei are observed.

28% of stage 14 egg chambers show persistant nurse cell nuclei, compared to 7% in controls.

(Bass et al., 2009)

Embryonic cells of mutant embryos do undergo apoptosis after exposure to X rays (as occurs in wild-type cells exposed to X rays) but the apoptosis is not accompanied by DNA fragmentation in the mutant cells.

(Manaka et al., 2004)

Apoptotic DNA fragmentation is not detectable in Rep1^P mutant embryos or adult ovaries. There is no abnormal deposition of acridine positive material in Rep1^P mutant embryos.

(Mukae et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

68% of Drep-1^P; DNaseII^lo double mutant stage 14 egg chambers show persisting nurse cell nuclei, enhanced compared to each single mutant phenotype. The persisting DNA is smeared as observed in DNaseII^lo mutants, unlike the discrete nuclei observed in wild-type and Drep-1^P single mutants. 3% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

(Bass et al., 2009)

Apoptotic DNA fragmentation is not detectable in Rep1^P, DNaseII^lo double mutant embryos or adult ovaries. The ovaries of double mutant Rep1^P, DNaseII^lo flies accumulate large amounts of acridine positive material.

(Mukae et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Drep-1^P

Drep1^P

Rep1_P

(Kuraishi et al., 2009)

Rep1^P

dICAD-null

(Manaka et al., 2004)

dICAD^P

(Bass et al., 2009)

p(icad)

(Mukae et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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