FlyBase Allele Report: Dmel\run[CK.UAS]

General Information

Symbol

Dmel\run^CK.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0143250

Feature type

allele

Associated gene

Dmel\run

Associated Insertion(s)

Carried in Construct

P{UAS-run.CK}

Also Known As

UAS-runt[CK]

Key Links

Transgenic product class

missense_variant

(Wheeler et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Wheeler et al., 2002)

Mutagen

in vitro construct

(Wheeler et al., 2002)

Progenitor genotype

run^UAS.cLa

(Wheeler et al., 2002)

Carried in construct

P{UAS-run.CK}

Cytology

Description

UAS regulatory sequences drive expression of a mutated run carrying the amino acid replacements K199A and C127S.

(Wheeler et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

run

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^MT14

(Walrad et al., 2010)

even pair-rule phenotype, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

lethal, with Scer\GAL4^nanos.PG

(Walrad et al., 2010)

lethal - all die during embryonic stage, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

odd pair-rule phenotype, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

Phenotype Manifest In

cephalopharyngeal skeleton, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

embryonic/larval cuticle, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

embryonic head, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

lamina plexus | third instar larval stage, with Scer\GAL4^MT14

(Walrad et al., 2010)

parasegment, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

ventral denticle belt, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

Detailed Description

Statement

Reference

Expression of run^CK.Scer\UAS under the control of Scer\GAL4^MT14 results in the disappearance of the lamina plexus in the optic lobe of larvae, due to the axons of photoreceptors R2-R5 terminating in the medulla (normally they terminate at the lamina plexus).

(Walrad et al., 2010)

Depending on the insertion line used, expressing run^CK.UAS under the control of Scer\GAL4^nos.PG results in a range of defects in head development and pair rule segmentation. As opposed to a strongly expressing insertion line, weak expression of run^CK.UAS only represses the initial activation of en-positive odd-numbered parasegments, which then recover during the extended germ band stage.

(Vander Zwan et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Kramer

(Wheeler et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

run^CK.Scer\UAS

run^CK.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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