FlyBase Allele Report: Dmel\run[UAS.cLa]

General Information

Symbol

Dmel\run^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Li

FlyBase ID

FBal0098662

Feature type

allele

Associated gene

Dmel\run

Associated Insertion(s)

Carried in Construct

P{UAS-run.L}

Also Known As

UAS-runt, P{UAS-runt.T}, UAS-run

Key Links

Transgenic product class

cDNA

(Tracey et al., 2000)

wild_type

(Tracey et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Tracey et al., 2000)

wild_type

(Tracey et al., 2000)

Mutagen

in vitro construct

(Tracey et al., 2000, Li and Gergen, 1999)

Progenitor genotype

Carried in construct

P{UAS-run.L}

Cytology

Description

UASt regulatory sequences drive expression of run sequences from 4 nucleotides upstream of the initiator methionine and extending through the polyadenylation signal to the poly(A) tail of a full length run cDNA.

(Tracey et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

run

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | gastrula stage | somatic clone, with Scer\GAL4^{VP16.mat.αTub67C}

(Zallen and Wieschaus, 2004)

abnormal cell shape | embryonic stage 6, with Scer\GAL4^{VP16.mat.αTub67C}

(Eritano et al., 2020)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^MT14

(Walrad et al., 2010)

even pair-rule phenotype, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

lethal, with Scer\GAL4^nanos.PG

(Walrad et al., 2010, Vanderzwan-Butler et al., 2007, Li and Gergen, 1999)

lethal | male, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

lethal - all die during embryonic stage, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

odd pair-rule phenotype, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

partially lethal, with Scer\GAL4^nanos.PG

(Wheeler et al., 2002)

partially lethal - majority die | female, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

some die during embryonic stage, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

visible, with Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

Phenotype Manifest In

cephalic furrow | absent | embryonic stage 6, with Scer\GAL4^{VP16.mat.αTub67C}

(Eritano et al., 2020)

cephalopharyngeal skeleton, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003, Tracey et al., 2000)

embryonic/larval cuticle, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

embryonic head, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

epithelial cell | embryonic stage 6, with Scer\GAL4^{VP16.mat.αTub67C}

(Eritano et al., 2020)

eye, with Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

germ band | gastrula stage | somatic clone, with Scer\GAL4^{VP16.mat.αTub67C}

(Zallen and Wieschaus, 2004)

lamina plexus | third instar larval stage, with Scer\GAL4^MT14

(Walrad et al., 2010)

ommatidium, with Scer\GAL4^GMR.PF

(Wildonger and Mann, 2005)

ommatidium, with Scer\GAL4^lz-gal4

(Wildonger and Mann, 2005)

parasegment, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

ventral denticle belt, with Scer\GAL4^nanos.PG

(Vander Zwan et al., 2003)

Detailed Description

Statement

Reference

Stage 6 embryos expressing run^UAS.cLa under the control of Scer\GAL4^{VP16.mat.αTub67C} often show reduced cell height at the cephalic furrow prospective site.

(Eritano et al., 2020)

Similar to wild type, midline glia are able to ensheath the axon commissures and extend membrane projections into the commissures in stage 15 embryos expressing run^Scer\UAS.cLa under the control of Scer\GAL4^sim.P3.7.

(Watson et al., 2011)

Expression of run^Scer\UAS.cLa under the control of Scer\GAL4^MT14 results in the disappearance of the lamina plexus in the optic lobe of larvae, due to the axons of photoreceptors R2-R5 terminating in the medulla (normally they terminate at the lamina plexus).

(Walrad et al., 2010)

Expression of run^Scer\UAS.cLa under the control of Scer\GAL4^nos.PG results in lethality in the blastoderm embryo.

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for Df(2L)C144 show a significant increase in viability from approximately 3% to approximately 100%.

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for Df(2L)JS32 show a significant increase in viability from approximately 3% to approximately 34%.

(Vanderzwan-Butler et al., 2007)

Expression of run^Scer\UAS.cLa under the control of Scer\GAL4^lz-gal4 or Scer\GAL4^GMR.PF results in eyes that appear glazed, due to the fact that individual ommatidia are virtually impossible to discern.

(Wildonger and Mann, 2005)

Anterior-posterior polarization of cells in the extending germband is disrupted in run^Scer\UAS.cLa; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} embryos. This disruption correlates with a failure of germband extension. (Note: The embryos analysed in these experiments were mosaics - due to the presence of lds^Hor-1, which induces the sporadic loss of paternally derived chromosomes).

(Zallen and Wieschaus, 2004)

Depending on the insertion line used, expressing run^UAS.cLa under the control of Scer\GAL4^nos.PG results in a range of defects in head development and pair rule segmentation. Specifically, run^CK.UAS represses the formation of en-positive odd-numbered parasegments.

(Vander Zwan et al., 2003)

Only 3% of run^Scer\UAS.cLa; Scer\GAL4^nos.PG animals survive to adulthood.

(Wheeler et al., 2002)

Expression of run^Scer\UAS.cLa under the control of Scer\GAL4^nos.PG results in embryos with defects along the anterior-posterior axis. Elimination of the entire head skeleton is also seen. Males are more sensitive than females to the lethal effects of expression of run^Scer\UAS.cLa under the control of Scer\GAL4^nos.PG.

(Tracey et al., 2000)

Expression of run^Scer\UAS.cLa under the control of Scer\GAL4^nos.PG results in partial to complete lethality, depending on the P{UAS-run.L} line used. Expression of run^Scer\UAS.cLa under the control of Scer\GAL4^hs.2sev results in a lz-like eye phenotype.

(Li and Gergen, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, run^UAS.cLa has visible phenotype, enhanceable by Bro::Hsap\MYH11^UAS.cLa, Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

Suppressed by

Statement

Reference

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal phenotype, suppressible by lilli^XS575

(Vanderzwan-Butler et al., 2007)

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal phenotype, suppressible by lilli^2L-193-35

(Vanderzwan-Butler et al., 2007)

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal phenotype, suppressible by lilli^7F1

(Vanderzwan-Butler et al., 2007)

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal phenotype, suppressible by lilli⁰⁰⁶³²

(Vanderzwan-Butler et al., 2007)

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal phenotype, suppressible by lilli^k05431

(Vanderzwan-Butler et al., 2007)

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal phenotype, suppressible by lilli^XS407

(Vanderzwan-Butler et al., 2007)

Scer\GAL4^nanos.PG, run^UAS.cLa has increased mortality during development phenotype, suppressible by ttk[+]/ttk⁰²⁶⁶⁷

(Vander Zwan et al., 2003)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, suppressible by CtBP[+]/CtBP⁰³⁴⁶³

(Wheeler et al., 2002)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, suppressible by Df(3L)GN50/+

(Wheeler et al., 2002)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, suppressible by +/Df(3R)ry615

(Wheeler et al., 2002)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, suppressible by gro^E48/gro[+]

(Wheeler et al., 2002)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, suppressible by ttk[+]/ttk^D2-50

(Wheeler et al., 2002)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, suppressible by HDAC1⁰⁴⁵⁵⁶/HDAC1[+]

(Wheeler et al., 2002)

Scer\GAL4^hs.2sev, run^UAS.cLa has visible phenotype, suppressible by Bro^UAS.cLa, Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^nanos.PG, run^UAS.cLa has increased mortality during development phenotype, non-suppressible by ttk^rM730

(Vander Zwan et al., 2003)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, non-suppressible by Df(3R)Espl22/+

(Wheeler et al., 2002)

Scer\GAL4^nanos.PG, run^UAS.cLa has partially lethal phenotype, non-suppressible by ttk[+]/ttk^rM730

(Wheeler et al., 2002)

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal | embryonic stage phenotype, non-suppressible by Sxl^f1

(Tracey et al., 2000)

Scer\GAL4^nanos.PG, run^UAS.cLa has lethal | embryonic stage phenotype, non-suppressible by Sxl^fP7B0

(Tracey et al., 2000)

Enhancer of

Statement

Reference

run^UAS.cLa, Scer\GAL4^hs.2sev is an enhancer of visible phenotype of Bro::Hsap\MYH11^UAS.cLa, Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

Other

Statement

Reference

Scer\GAL4^nanos.PG, run^UAS.cLa, ttk[+]/ttk^osn has lethal phenotype

(Vander Zwan et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, run^UAS.cLa has eye phenotype, enhanceable by Bro::Hsap\MYH11^UAS.cLa, Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, run^UAS.cLa has eye phenotype, suppressible by Bro^UAS.cLa, Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

Enhancer of

Statement

Reference

run^UAS.cLa, Scer\GAL4^hs.2sev is an enhancer of eye phenotype of Bro::Hsap\MYH11^UAS.cLa, Scer\GAL4^hs.2sev

(Li and Gergen, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for lilli⁰⁰⁶³² show a significant increase in viability from approximately 3% to approximately 33%.

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for lilli^k05431 show a significant increase in viability from approximately 3% to approximately 35%.

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for lilli^XS407 show a significant increase in viability from approximately 3% to approximately 28%.

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for lilli^XS575 show a significant increase in viability from approximately 3% to approximately 51%.

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for lilli^2L-193-35 show a slight increase in viability from approximately 3% to approximately 5%.

run^Scer\UAS.cLa (Scer\GAL4^nos.PG) embryos that are also heterozygous for lilli^7F1 show a slight increase in viability from approximately 3% to approximately 32%.

(Vanderzwan-Butler et al., 2007)

The partial lethality due to run^Scer\UAS.cLa; Scer\GAL4^nos.PG (3% viable) is partially suppressed by maternal heterozygosity for the following: gro^E48 (rescues to 30% viable); CtBP⁰³⁴⁶³ (rescues to 15% viable); ttk^1e11 (rescues to 21% viable); Rpd3⁰⁴⁵⁵⁶ (rescues to 31% viable); Df(3R)ry615 (rescues to 29% viable); Df(3L)GN50 (rescues to 36% viable); Df(3R)faf-BP (rescues to 40% viable); ttk¹ (rescues to 19% viable); ttk⁵³⁴⁶ (rescues to 58% viable); ttk^B330 (rescues to 33% viable) ttk¹⁰⁵⁵⁶ (rescues to 27% viable); ttk^D2-50 (rescues to 8% viable). The partial lethality due to run^Scer\UAS.cLa; Scer\GAL4^nos.PG (3% viable) is not suppressed by maternal heterozygosity for ttk^rM730, ttk^osn, or Df(3R)Espl22.

(Wheeler et al., 2002)

The preferential sensitivity of males to the lethal effects of expression of run^Scer\UAS.cLa under the control of Scer\GAL4^nos.PG is not suppressed by Sxl^f1 or Sxl^fP7B0.

(Tracey et al., 2000)

Xenogenetic Interactions

Statement

Reference

The eye phenotype caused by expression of run^Scer\UAS.cLa under the control of Scer\GAL4^hs.2sev is suppressed by coexpression of Bro^Scer\UAS.cLa. A severe eye phenotype is produced when run^Scer\UAS.cLa and Bro::Hsap\MYH11^Scer\UAS.cLa are coexpressed under the control of Scer\GAL4^hs.2sev.

(Li and Gergen, 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

run^Scer\UAS.cLa

run^Scer\UAS.cTa

run^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Li

(Li and Gergen, 1999)

Saccharomyces cerevisiae UAS construct a of Tracey

(Tracey et al., 2000)

Secondary FlyBase IDs

FBal0103580

References (18)

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