FlyBase Allele Report: Dmel\mts[dn181.UAS]

General Information

Symbol

Dmel\mts^dn181.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0143316

Feature type

allele

Associated gene

Dmel\mts

Associated Insertion(s)

Carried in Construct

P{UAS-mts.dn181}

Key Links

Transgenic product class

dominant_negative_variant

(Hannus et al., 2002)

inframe_deletion

(Hannus et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Hannus et al., 2002)

inframe_deletion

(Hannus et al., 2002)

Mutagen

in vitro construct

(Hannus et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-mts.dn181}

Cytology

Description

UASt regulatory sequences drive expression of a dominant negative form of mts (an N-terminally truncated form starting at amino acid 181).

(Hannus et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mts

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Sathyanarayanan et al., 2004)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Sathyanarayanan et al., 2004)

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Viquez et al., 2006)

abnormal planar polarity, with Scer\GAL4^ptc-559.1

(Hannus et al., 2002)

increased cell number, with Scer\GAL4^Act.PU

(Chabu and Doe, 2009)

Phenotype Manifest In

bouton & neuromuscular junction | larval stage, with Scer\GAL4^elav.PLu

(Viquez et al., 2006)

larval neuroblast, with Scer\GAL4^Act.PU

(Chabu and Doe, 2009)

microtubule & neuromuscular junction | larval stage, with Scer\GAL4^elav.PLu

(Viquez et al., 2006)

wing hair, with Scer\GAL4^ptc-559.1

(Hannus et al., 2002)

Detailed Description

Statement

Reference

mts^{dn181.Scer\UAS} under the control of Scer\GAL4^Act results in an increase in neuroblast numbers compared to controls.

(Chabu and Doe, 2009)

Larvae expressing mts^{dn181.Scer\UAS} under the control of Scer\GAL4^elav.PLu have aberrant bouton morphology and size, and disruption to the NMJ cytoskeleton, with an 88% increase in the levels of unbundled microtubules relative to wild-type. Boutons are observed filled with an unusually high number of splayed filaments.

(Viquez et al., 2006)

Flies expressing mts^{dn181.Scer\UAS} under the control of Scer\GAL4^tim.PE are arrhythmic with respect to locomotor activity. Flies expressing mts^{dn181.Scer\UAS} under the control of Scer\GAL4^P2.4.Pdf show an increase in the period of locomotor activity rhythms compared to controls. The rhythms appear to be unstable; some flies have long periods with interspersed days of prolonged activity and others show multiple periodicities.

(Sathyanarayanan et al., 2004)

Expression of mts^{dn181.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 results in a lack of wing hairs over a significant region of the anterior-posterior compartment boundary. In regions where hairs are present, they often have abnormal orientation.

(Hannus et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, mts^dn181.UAS has abnormal neuroanatomy phenotype, enhanceable by PP2A-B'[+]/wrd¹⁰⁴/Scer\GAL4^elav.PLu

(Viquez et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, mts^dn181.UAS has microtubule & neuromuscular junction | larval stage phenotype, enhanceable by PP2A-B'[+]/wrd¹⁰⁴/Scer\GAL4^elav.PLu

(Viquez et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

A PP2A-B'¹⁰⁴ heterozygote background significantly enhances the unbundling microtubule phenotype of neuronal mts^{dn181.Scer\UAS} expression (under the control of Scer\GAL4^elav.PLu), leading to a 46% increase in unbundled microtubules relative to mts^{dn181.Scer\UAS} expression alone. In addition, the cytoskeletal morphology of these double mutants is more severely disrupted than in mts^{dn181.Scer\UAS} mutants. Simultaneously inhibiting mts and PP2A-B' results in synapses in which microtubules are splayed or punctate through the normally stable core region of the synaptic terminal.

(Viquez et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

mts^DN181

(Andreazza et al., 2015)

mts^DN

(Andreazza et al., 2015)

mts^{dn181.Scer\UAS}

mts^dn181.UAS

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close