The genomic rescue construct P{3GBix.mut} is derived from P{2GB}, by deletion of a fragment including the ORFs of tRNA:SeC-TCA-1-1 and ιTry, and by mutagenesis of ix such that codon 91 is changed to a stop. CG12384 and san ORFs remain intact.
ixt3GBix.mut fails to rescue ix2