FlyBase Allele Report: Dmel\Sod2[RNAi.UAS.cKa]

General Information

Symbol

Dmel\Sod2^RNAi.UAS.cKa

Species

D. melanogaster

Name

FlyBase ID

FBal0144561

Feature type

allele

Associated gene

Dmel\Sod2

Associated Insertion(s)

Carried in Construct

P{UAS-Sod2.RNAi.K}

Also Known As

UAS-Sod2IR, SOD2_dsRNA, Sod2^RNAi

Key Links

Transgenic product class

RNAi_reagent

(Kirby et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kirby et al., 2002)

Mutagen

in vitro construct

(Kirby et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Sod2.RNAi.K}

Cytology

Description

Template consists of an inverted repeat of a 757bp fragment of Sod2 cDNA (from the 3' end of the cDNA (minus the PolyA signal) to a 5' EcoRI site). Expression is driven by UASt sequences.

(Kirby et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Sod2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^Aβ42.lexAop

(Stapper and Jahn, 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived, with Scer\GAL4^da.G32

(Wicks et al., 2009, Kirby et al., 2002)

short lived, with Scer\GAL4^da.PU

(Filograna et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Sod2^{dsRNA.Scer\UAS.cKa} RNAi under the control of Scer\GAL4^da.PU strongly reduces the survival rate of adult flies compared to controls.

(Filograna et al., 2016)

Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2^Scer\UAS.cDa) does not significantly affect circadian rhythmicity in locomotor activity assays in constant darkness.

(Mandilaras and Missirlis, 2012)

Chronic hypoxia (5% O[[2]]) dramatically restores the abbreviated life span of flies expressing Sod2^{dsRNA.Scer\UAS.cKa} under the control of Scer\GAL4^da.G32.

Survival of flies expressing Sod2^{dsRNA.Scer\UAS.cKa} under the control of Scer\GAL4^da.G32 rapidly plummets from well over 90% to less than 25% within a day when the flies are transferred from permissive hypoxia to restrictive normoxia conditions. If flies expressing Sod2^{dsRNA.Scer\UAS.cKa} under the control of Scer\GAL4^da.G32 are transferred from normoxia to hypoxia, the high mortality of the flies rapidly arrests, and the survivors live as long as their cohorts maintained under permanent hypoxia.

(Wicks et al., 2009)

Extracts from Sod2^{dsRNA.Scer\UAS.cKa}; Scer\GAL4^da.G32 flies have reduced mitochondrial aconitase activity, and reduced succinate dehydrogenase activity. aconitase activity can be recovered by treating the extract with a iron and a thiol reductant. Sod2^{dsRNA.Scer\UAS.cKa}; Scer\GAL4^da.G32 flies are hypersensitive to paraquat toxicity (survival is reduced to 2% of control levels with P{UAS-Sod2.dsRNA.K}24 and 17 % of control levels with P{UAS-Sod2.dsRNA.K}15 on exposure to 2mM paraquat. Pre-adult mortality in Sod2^{dsRNA.Scer\UAS.cKa}; Scer\GAL4^da.G32 animals is no different to wild-type, but rapid onset adult mortality in these flies leads to a severe reduction in average lifespan (86% reduction with P{UAS-Sod2.dsRNA.K}15; 76% reduction with P{UAS-Sod2.dsRNA.K}24.)

(Kirby et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Sod2^RNAi.UAS.cKa/Scer\GAL4^Mef2.PR is a suppressor of long lived phenotype of Scer\GAL4^Mef2.PR, p38b^UAS.cAa

(Vrailas-Mortimer et al., 2011)

NOT Suppressor of

Statement

Reference

Sod2^RNAi.UAS.cKa, Scer\GAL4^Hml.Δ is a non-suppressor of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Sod2^RNAi.UAS.cKa, Scer\GAL4^Hml.Δ is a non-suppressor of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Sod2^{dsRNA.Scer\UAS.cKa} suppresses the lifespan extension phenotype seen when p38b^Scer\UAS.cAa is expressed in muscles under the control of Scer\GAL4^Mef2.PR.

(Vrailas-Mortimer et al., 2011)

Expression of Sod2^{dsRNA.Scer\UAS.cKa} does not suppress the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24489

w¹; P{UAS-Sod2.RNAi.K}15/SM5

Notes on Origin

Discoverer

Comments

dsRNA is expressed from the template to study the phenotypic consequences of dsRNA interference (RNAi) of the Sod2 gene.

(Kirby et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sod2^RNAi.UAS.cKa

Sod2^{dsRNA.Scer\UAS.cKa}

Sod2^{dsRNA.UAS.cKa}

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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