FlyBase Allele Report: Dmel\rols[08232ex18]

General Information

Symbol

Dmel\rols^08232ex18

Species

D. melanogaster

Name

FlyBase ID

FBal0146840

Feature type

allele

Associated gene

Dmel\rols

Associated Insertion(s)

Carried in Construct

Also Known As

P1729ex18

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Menon and Chia, 2001)

Progenitor genotype

rols⁰⁸²³²

(Menon and Chia, 2001)

Cytology

Description

Imprecise excision of P{PZ}rols⁰⁸²³² has deleted approximately 2kB either side of the original insertion.

(Menon and Chia, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(3L)BK9)

(Menon et al., 2005)

Phenotype Manifest In

dorsal acute muscle & myotube

(Menon and Chia, 2001)

muscle cell of A1-7 dorsal acute muscle 1 | embryonic stage 15 (with Df(3L)BK9)

(Menon et al., 2005)

presumptive embryonic/larval muscle system (with Df(3L)BK9)

(Menon et al., 2005)

Detailed Description

Statement

Reference

Significant myoblast fusion fails to occur in rols^08232ex18/Df(3L)BK9 embryos - the average number of eve-positive nuclei within a DA1 muscle at stage 15 is 3.3 compared to 9.9 in wild type embryos. Most rols^08232ex18/Df(3L)BK9 embryos fail to hatch and eventually die.

(Menon et al., 2005)

While a wild-type DA1 myotube has an average of 15 nuclei at stage 15 (n = 70), its equivalent in embryos homozygous for rols^08232ex18 has an average of just 2 (n = 108).

(Menon and Chia, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3L)BK9/rols^08232ex18 is rescued by rols^7.UAS/Scer\GAL4^how-24B

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is rescued by Scer\GAL4^how-24B/rols^UAS.N.Tag:HA

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is rescued by rols^UAS.C.Tag:HA/Scer\GAL4^how-24B

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is rescued by Scer\GAL4^how-24B/rols^{Δ1-137.UAS.Tag:HA}

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is rescued by Scer\GAL4^how-24B/rols^{Δ1-264.UAS.Tag:HA}

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is rescued by Scer\GAL4^how-24B/rols^{Δ1-439.UAS.Tag:HA}

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is rescued by Scer\GAL4^how-24B/rols^{S241A.S243A.UAS}

(Menon et al., 2005)

Partially rescued by

Df(3L)BK9/rols^08232ex18 is partially rescued by Scer\GAL4^how-24B/rols^{Δ1739-1900.UAS.Tag:HA}

(Menon et al., 2005)

Not rescued by

Df(3L)BK9/rols^08232ex18 is not rescued by Scer\GAL4^how-24B/rols^6.UAS

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is not rescued by Scer\GAL4^how-24B/rols^{Δ1-863.UAS.Tag:HA}

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is not rescued by rols^{Δ1-1150.UAS.Tag:HA}/Scer\GAL4^how-24B

(Menon et al., 2005)

Df(3L)BK9/rols^08232ex18 is not rescued by Scer\GAL4^how-24B/rols^{Δ1401-1900.UAS.Tag:HA}

(Menon et al., 2005)

Comments

Expression of rols^6.Scer\UAS, rols^{Δ1-863.Scer\UAS.T:Ivir\HA1}, rols^{Δ1-1150.Scer\UAS.T:Ivir\HA1} or rols^{Δ1401-1900.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^how-24B fails to rescue the myoblast fusion defect of rols^08232ex18/Df(3L)BK9 embryos.

Expression of rols^7.Scer\UAS, rols^{Scer\UAS.N.T:Ivir\HA1}, rols^{Scer\UAS.C.T:Ivir\HA1}, rols^{Δ1-137.Scer\UAS.T:Ivir\HA1}, rols^{Δ1-264.Scer\UAS.T:Ivir\HA1}, rols^{Δ1-439.Scer\UAS.T:Ivir\HA1} or rols^{S241A.S243A.Scer\UAS} under the control of Scer\GAL4^how-24B fully rescues the myoblast fusion defect of rols^08232ex18/Df(3L)BK9 embryos.

Expression of rols^{Δ1739-1900.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^how-24B partially rescues the myoblast fusion defect of rols^08232ex18/Df(3L)BK9 embryos. However, a functional musculature is not produced, most of the embryos fail to hatch and eventually die.

(Menon et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

1729ex18

(Menon et al., 2005)

P1729ex18

(Menon et al., 2005, Menon and Chia, 2001)

rols^08232ex18

rols^1729ex18

(Menon et al., 2005)

rols^P1729ex18

(Menon et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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