FlyBase Allele Report: Dmel\p53[UAS.cPa]

General Information

Symbol

Dmel\p53^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Peters

FlyBase ID

FBal0146953

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{UAS-p53.P}

Also Known As

UAS-Dmp53

Key Links

Transgenic product class

wild_type

(Peters et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Peters et al., 2002)

Mutagen

in vitro construct

(Peters et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-p53.P}

Cytology

Description

UAS regulatory sequences drive expression of p53.

(Peters et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^GMR.PF

(Peters et al., 2002)

visible, with Scer\GAL4^GMR.PF

(Peters et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Peters et al., 2002)

eye disc, with Scer\GAL4^GMR.PF

(Peters et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Peters et al., 2002)

ommatidium, with Scer\GAL4^GMR.PF

(Peters et al., 2002)

Detailed Description

Statement

Reference

Expression of p53^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF results in a reduced eye, with partial fusion of ommatidia and some loss of bristles. Eye discs show extensive apoptosis. There are no detectable effects on cell cycle distribution or cell size in the eye disc compared to wild type.

(Peters et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, enhanceable by Scer\GAL4^GMR.PF/lok^UAS.cPa

(Peters et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has increased cell death phenotype, non-enhanceable by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, non-enhanceable by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, non-enhanceable by grp[+]/grp⁰⁶⁰³⁴

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has increased cell death phenotype, non-enhanceable by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, non-enhanceable by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has increased cell death phenotype, suppressible by Scer\GAL4^GMR.PF/lok^D303A.UAS

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, suppressible by Scer\GAL4^GMR.PF/lok^D303A.UAS

(Peters et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, non-suppressible by grp[+]/grp⁰⁶⁰³⁴

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has increased cell death phenotype, non-suppressible by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has increased cell death phenotype, non-suppressible by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, non-suppressible by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has visible phenotype, non-suppressible by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Other

Statement

Reference

Scer\GAL4^GMR.PF, lok^UAS.cPa, p53^UAS.cPa has increased cell death phenotype

(Peters et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, enhanceable by Scer\GAL4^GMR.PF/lok^UAS.cPa

(Peters et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, non-enhanceable by grp[+]/grp⁰⁶⁰³⁴

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye disc phenotype, non-enhanceable by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, non-enhanceable by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye disc phenotype, non-enhanceable by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, non-enhanceable by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, suppressible by Scer\GAL4^GMR.PF/lok^D303A.UAS

(Peters et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, non-suppressible by grp[+]/grp⁰⁶⁰³⁴

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye disc phenotype, non-suppressible by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye disc phenotype, non-suppressible by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, non-suppressible by grp^D130A.UAS/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.cPa has eye phenotype, non-suppressible by grp^UAS.cPa/Scer\GAL4^GMR.PF

(Peters et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of lok^Scer\UAS.cPa and p53^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF results in almost complete loss of the eye. Excessive apoptosis is seen in third larval instar eye discs in these animals. Co-expression of lok^{D303A.Scer\UAS} almost completely rescues the eye phenotype caused by expression of p53^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF. No apoptosis is found in the eye discs of third instar larvae. Co-expression of either grp^Scer\UAS.cPa or grp^{D130A.Scer\UAS} does not alter the eye phenotype or number of apoptotic cells in the larval eye disc caused by expression of p53^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF. No apoptosis is found in the eye discs of third instar larvae.

(Peters et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "pUAST-dm-p53", transfected into S2 cells with a driver construct and used in transcription activation assays.

(Peters et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

p53^Scer\UAS.cPa

p53^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Peters

(Peters et al., 2002)

Secondary FlyBase IDs

References (2)

Report Sections

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