FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\lokD303A.UAS
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General Information
Symbol
Dmel\lokD303A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0147040
Feature type
allele
Associated gene
Dmel\lok
Associated Insertion(s)
Carried in Construct
P{UAS-lok.D303A}
Key Links
Transgenic product class
dominant_negative_variant
(Peters et al., 2002)
missense_variant
(Peters et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Peters et al., 2002)
missense_variant
(Peters et al., 2002)
Mutagen
in vitro construct
(Peters et al., 2002)
Progenitor genotype
lokUAS.cPa
(Peters et al., 2002)
Carried in construct
P{UAS-lok.D303A}
Cytology
Description

UAS regulatory sequences drive expression of a dominant-negative form of lok (carries the amino acid replacement D303A).

(Peters et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
lok
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of lokD303A.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in an overt phenotype in the eye and no apoptotic cells are detected in third larval instar eye discs.

      (Peters et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      Scer\GAL4GMR.PF/lokD303A.UAS is a non-enhancer of visible phenotype of Scer\GAL4GMR.PF, p53S4A.UAS

      (Peters et al., 2002)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4GMR.PF/lokD303A.UAS is a non-suppressor of visible phenotype of Scer\GAL4GMR.PF, p53S4A.UAS

      (Peters et al., 2002)
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      Scer\GAL4GMR.PF/lokD303A.UAS is a non-enhancer of eye phenotype of Scer\GAL4GMR.PF, p53S4A.UAS

      (Peters et al., 2002)
      Suppressor of
      Statement
      Reference

      Scer\GAL4GMR.PF/lokD303A.UAS is a suppressor of eye phenotype of Scer\GAL4GMR.PF, p53UAS.cPa

      (Peters et al., 2002)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4GMR.PF/lokD303A.UAS is a non-suppressor of eye phenotype of Scer\GAL4GMR.PF, p53S4A.UAS

      (Peters et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of lokD303A.Scer\UAS almost completely rescues the eye phenotype caused by expression of p53Scer\UAS.cPa under the control of Scer\GAL4GMR.PF. No apoptosis is found in the eye discs of third instar larvae. The small eye phenotype caused by expression of p53S4A.Scer\UAS under the control of Scer\GAL4GMR.PF is not altered by coexpression of lokD303A.Scer\UAS.

      (Peters et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "pUAST-dm-chk2-DN", transfected into S2 cells with a driver construct and used in transcription activation assays.

      (Peters et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      lokD303A.Scer\UAS
      lokD303A.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)