FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\hhC85S.UAS.cGa
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General Information
Symbol
Dmel\hhC85S.UAS.cGa
Species
D. melanogaster
Name
FlyBase ID
FBal0150434
Feature type
allele
Associated gene
Dmel\hh
Associated Insertion(s)
Carried in Construct
P{UAS-hh.C85S.G}
Key Links
Transgenic product class
missense_variant
(Gallet et al., 2003)
polypeptide_post_translational_processing_variant
(Gallet et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Gallet et al., 2003)
polypeptide_post_translational_processing_variant
(Gallet et al., 2003)
Mutagen
in vitro construct
(Gallet et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-hh.C85S.G}
Cytology
Description

UASt regulatory sequences drive expression of hh which has been mutated to carry the C85S amino acid replacement. This is expected to result in a protein which can be processed by autoproteolytic cleavage and modified at the C-terminal end by the addition of cholesterol, but which cannot undergo palmitoylation at the N-terminal end.

(Gallet et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
hh
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ectopic expression of hhC85S.Scer\UAS.cGa, under the control of Scer\GAL4unspecified in hhAC first instar larvae induces only type-2 cuticle, and no type 3 cuticle.

      hhC85S.Scer\UAS.cGa expressing cells do not display any membrane staining.

      Approximately 80% of wing discs containing hhC85S.Scer\UAS.cGa flip-out clones (under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C)display anterior compartment outgrowth.

      (Gallet et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of hhC85S.Scer\UAS.cGa, under the control of Scer\GAL4unspecified in dispS037707 mutant embryos, some type 2, but no type 3 cuticle is formed.

      (Gallet et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      hhC85S.UAS.cGa/Scer\GAL469B partially rescues hhAC

      (Gallet et al., 2003)
      Comments

      Naked cuticle formation in hhAC/hhAC embryonic cuticle is rescued by hhC85S.Scer\UAS; Scer\GAL469B, and the denticle belts formed are almost normal (row 2 is abnormal, and rows 5 and 6 are often incomplete).

      (Gallet et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      hhC85S.Scer\UAS.cGa
      hhC85S.UAS.cGa
      Name Synonyms
      Secondary FlyBase IDs
        References (3)