FlyBase Allele Report: Dmel\vib[EP651]

General Information

Symbol

Dmel\vib^EP651

Species

D. melanogaster

Name

FlyBase ID

FBal0151355

Feature type

allele

Associated gene

Dmel\vib

Associated Insertion(s)

P{EP}vib^EP651

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bidet et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{EP}vib^EP651

Cytology

Description

Insertion 222 bp upstream of the first exon (i.e. outside of the transcribed region) and oriented in a way that should promote expression of vib.

(Gatt and Glover, 2006)

P{EP} insertion 222bp upstream of the vib translation initiation codon. The P{EP} element is oriented such that expression from the element should result in overexpression of vib.

(Bidet et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:19,226,667..19,226,667 [-]

(Branco et al., 2008, Gatt and Glover, 2006, Bidet et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

ameliorates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile (with Df(3R)Delta-BX12)

(Gatt and Glover, 2006)

lethal - all die during embryonic stage

(Gatt and Glover, 2006)

some die during embryonic stage

(Gatt and Glover, 2006)

viable (with Df(3R)Delta-BX12)

(Gatt and Glover, 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

No retinal phenotypes are seen when vib^EP651 is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

vib^EP651/Df(3R)Dl-BX12 mutant flies are phenotypically normal.

(Gatt and Glover, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

vib^EP651, Scer\GAL4^nrv2.PS is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^nrv2.PS

(Branco et al., 2008)

Suppressor of

Statement

Reference

vib^EP651, Scer\GAL4^nrv2.PS is a suppressor | partially of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^nrv2.PS

(Branco et al., 2008)

vib^EP651, Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

vib^EP651, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Suppressor of

Statement

Reference

vib^EP651, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

vib^EP651, Scer\GAL4^GMR.PU is a suppressor of ommatidium phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

vib^EP651, Scer\GAL4^GMR.PU is a suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of vib^EP651 enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of vib^EP651 suppresses the external eye and retinal phenotypes seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

Expression of vib^EP651 partially suppresses the climbing defects seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^nrv2.PS.

Expression of vib^EP651 enhances the climbing defects seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^nrv2.PS.

Expression of vib^EP651 reduces the number of neurons that contain nuclear inclusions seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^ey-OK107.

Expression of vib^EP651 increases the number of neurons that contain nuclear inclusions seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^ey-OK107.

(Branco et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: A second site mutation causing early embryonic lethality.

(Gatt and Glover, 2006)

Comments

Based on the lethal stage of hemizygous alleles (over Df(3R)Dl-BX12), the following vib alleles can be ranked from strongest to weakest as follows: vib^j7A3 > vib^S110416 > vib^EP513 = vib^j5A6 > vib^S045002c > vib^EP651.

(Gatt and Glover, 2006)

FlyBase Curator comment: FBrf0194258 states P{EP}vib^EP651 is inserted 222 bp upstream of the vib transcription start site, whereas FBrf0173131 states it is 222bp upstream of the translation start site.

(Gatt and Glover, 2006, Bidet et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

vib^EP651

(Gatt and Glover, 2006)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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