FlyBase Allele Report: Mmus\Has2[UAS.cTa]

General Information

Symbol

Mmus\Has2^UAS.cTa

Species

M. musculus

Name

Saccharomyces cerevisiae UAS construct a of Takeo

FlyBase ID

FBal0156234

Feature type

allele

Associated gene

Mmus\Has2

Associated Insertion(s)

Carried in Construct

P{UAS-HAS2.T}

Transgenic product class

cDNA

(Takeo et al., 2004)

wild_type

(Takeo et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Takeo et al., 2004)

wild_type

(Takeo et al., 2004)

Mutagen

in vitro construct

(Takeo et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-HAS2.T}

Cytology

Description

UASt regulatory sequences drive expression of a Mmus\Has2 cDNA.

(Takeo et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Has2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^29BD

(Takeo et al., 2004)

lethal, with Scer\GAL4^Act5C.PU

(Takeo et al., 2004)

visible, with Scer\GAL4^29BD

(Takeo et al., 2004)

visible, with Scer\GAL4^A9

(Takeo et al., 2004)

visible, with Scer\GAL4^ap-md544

(Takeo et al., 2004)

visible, with Scer\GAL4^en-e16E

(Takeo et al., 2004)

visible, with Scer\GAL4^GMR.PF

(Takeo et al., 2004)

Phenotype Manifest In

cone cell, with Scer\GAL4^GMR.PF

(Takeo et al., 2004)

eye, with Scer\GAL4^GMR.PF

(Takeo et al., 2004)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Takeo et al., 2004)

macrochaeta, with Scer\GAL4^ap-md544

(Takeo et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Takeo et al., 2004)

primary pigment cell, with Scer\GAL4^GMR.PF

(Takeo et al., 2004)

wing, with Scer\GAL4^29BD

(Takeo et al., 2004)

wing, with Scer\GAL4^ap-md544

(Takeo et al., 2004)

wing, with Scer\GAL4^en-e16E

(Takeo et al., 2004)

wing vein, with Scer\GAL4^29BD

(Takeo et al., 2004)

wing vein, with Scer\GAL4^A9

(Takeo et al., 2004)

wing vein, with Scer\GAL4^en-e16E

(Takeo et al., 2004)

Detailed Description

Statement

Reference

Flies expressing Mmus\Has2^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF have a rough eye phenotype, with reduced numbers of ommatidia and disordered ommatidial arrays. The number of cone cells is decreased, the primary pigment cells have abnormal sizes and shapes and ectopic interommatidial bristles are seen. Flies expressing Mmus\Has2^Scer\UAS.cTa under the control of Scer\GAL4^A9 have thickened and disarranged wing veins. The wings fail to extend in flies expressing Mmus\Has2^Scer\UAS.cTa under the control of Scer\GAL4^ap-md544 and most macrochaetae on the notum are missing or are shorter than normal with abruptly ended tips. Animals expressing Mmus\Has2^Scer\UAS.cTa under the control of Scer\GAL4^Act5C.PI die during the first larval instar or pupal stage (depending on the P{UAS-HAS2.T} line used). Animals expressing Mmus\Has2^Scer\UAS.cTa under the control of Scer\GAL4^29BD show lethality at the pupal stage when P{UAS-HAS2.T}1 is used. When P{UAS-HAS2.T}2 is used at 20^oC, the flies have disordered wing veins, the wings have a higher water content than normal and the flies have an eclosion defect. Animals expressing Mmus\Has2^Scer\UAS.cTa under the control of Scer\GAL4^en-e16E show an eclosion defect and have folded wings when P{UAS-HAS2.T}1 is used and have disordered wing veins and wings with a higher water content than normal when P{UAS-HAS2.T}2 is used.

(Takeo et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mmus\Has2^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by sgl^Ubi-p63E.PH

(Takeo et al., 2004)

NOT Enhanced by

Statement

Reference

Mmus\Has2^UAS.cTa, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by sgl^unspecified

(Takeo et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mmus\Has2^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by sgl^Ubi-p63E.PH

(Takeo et al., 2004)

NOT Enhanced by

Statement

Reference

Mmus\Has2^UAS.cTa, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by sgl^unspecified

(Takeo et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Mmus\Has2^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF is not affected by sgl^unspecified, but is dramatically enhanced by sgl^Ubi-p63E.PH.

(Takeo et al., 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mmus\Has2^Scer\UAS.cTa

Mmus\Has2^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Takeo

(Takeo et al., 2004)

Secondary FlyBase IDs

References (1)

Report Sections

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