FlyBase Allele Report: Dmel\Egfr::kek1[KEgG.UAS.GFP]

General Information

Symbol

Dmel\Egfr::kek1^KEgG.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0156486

Feature type

allele

Associated gene

Egfr::kek1

Associated Insertion(s)

Carried in Construct

P{UAS-kek1::Egfr.KEgG}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Alvarado et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-kek1::Egfr.KEgG}

Cytology

Description

UAS regulatory sequences drive expression of the extracellular and transmembrane domains of kek1 fused to the entire Egfr cytoplasmic domain. A G901R mutation (which disrupts ATP binding) has been introduced within the Egfr kinase domain, abolishing kinase activity. The chimeric protein is tagged at the C-terminal end with GFP.

(Alvarado et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Egfr::kek1

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

Phenotype Manifest In

egg chorion, with Scer\GAL4^CY2

(Alvarado et al., 2004)

eye, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

Detailed Description

Statement

Reference

Expression of Egfr::kek1^{KEgG.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^CY2 results in eggs with ventralised chorions. Expression of Egfr::kek1^{KEgG.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Alvarado et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Egfr::kek1^KEgG.UAS.GFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Egfr[+]/Egfr^SOK2

(Alvarado et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Egfr::kek1^KEgG.UAS.GFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Egfr[+]/Egfr^SOK2

(Alvarado et al., 2004)

Egfr::kek1^KEgG.UAS.GFP, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Egfr[+]/Egfr^SOK2

(Alvarado et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Egfr::kek1^{KEgG.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF is strongly suppressed by Egfr^SOK2/+.

(Alvarado et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Egfr::kek1^{KEgG.Scer\UAS.T:Avic\GFP}

Egfr::kek1^KEgG.UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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