FlyBase Allele Report: Dmel\Dap160[Δ1]

General Information

Symbol

Dmel\Dap160^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0160635

Feature type

allele

Associated gene

Dmel\Dap160

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Koh et al., 2004)

Progenitor genotype

Dap160^EP2468

(Koh et al., 2004)

Cytology

Description

Imprecise excision of the P{EP} element.

(Koh et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cJa

(Spargo et al., 2025)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with Dap160^Q24)

(Chabu and Doe, 2008)

abnormal neuroanatomy (with Dap160^Δ2)

(Koh et al., 2004)

abnormal neuroanatomy (with Dap160^Δ3)

(Koh et al., 2004)

abnormal neuroanatomy | larval stage (with Dap160^EMS)

(O'Connor-Giles et al., 2008)

abnormal neuroanatomy | larval stage (with Df(2L)bur-K1)

(Koh et al., 2007)

abnormal neuroanatomy | third instar larval stage (with Dap160^Q24)

(Chabu and Doe, 2008)

abnormal neurophysiology (with Dap160^Δ2)

(Koh et al., 2004)

abnormal neurophysiology (with Dap160^Δ3)

(Koh et al., 2004)

abnormal neurophysiology | larval stage (with Df(2L)bur-K1)

(Koh et al., 2007)

lethal - all die before end of P-stage (with Dap160^Δ2)

(Koh et al., 2004)

lethal - all die before end of P-stage (with Df(2L)bur-K1)

(Koh et al., 2004)

paralytic | heat sensitive (with Dap160^EMS)

(Koh et al., 2004)

paralytic | heat sensitive (with Dap160^Δ6)

(Koh et al., 2004)

some die during pharate adult stage (with Dap160^Δ2)

(Koh et al., 2004)

some die during pharate adult stage (with Df(2L)bur-K1)

(Koh et al., 2004)

uncoordinated | adult stage (with Dap160^EMS)

(Koh et al., 2004)

uncoordinated | adult stage (with Dap160^Δ3)

(Koh et al., 2004)

uncoordinated | adult stage (with Dap160^Δ6)

(Koh et al., 2004)

Phenotype Manifest In

bouton (with Dap160^Δ2)

(Koh et al., 2004)

bouton (with Dap160^Δ3)

(Koh et al., 2004)

bouton (with Df(2L)bur-K1)

(Koh et al., 2007)

bouton | increased number (with Dap160^Δ2)

(Koh et al., 2004)

bouton | increased number (with Dap160^Δ3)

(Koh et al., 2004)

embryonic/larval neuromuscular junction (with Df(2L)bur-K1)

(Koh et al., 2007)

larval neuroblast (with Dap160^Q24)

(Chabu and Doe, 2008)

larval neuroblast & larval brain (with Dap160^Q24)

(Chabu and Doe, 2008)

neuromuscular junction (with Dap160^Δ2)

(Koh et al., 2004)

neuromuscular junction (with Dap160^Δ3)

(Koh et al., 2004)

NMJ bouton | larval stage | increased number (with Dap160^EMS)

(O'Connor-Giles et al., 2008)

synaptic vesicle (with Dap160^Δ2)

(Koh et al., 2004)

Detailed Description

Statement

Reference

Dap160^Q24/Dap160^Δ1 third instar larvae have a reduced number of neuroblasts per brain lobe compared to wild-type controls.

Progression through mitosis is delayed in Dap160^Q24/Dap160^Δ1 larval neuroblasts compared to wild-type neuroblasts; the transit from nuclear envelope breakdown to anaphase onset is 13.37 +/- 4.4 minutes in the mutant neuroblasts compared to 7.76 +/- 2.04 minutes in wild-type neuroblasts.

(Chabu and Doe, 2008)

The levels of membrane uptake (dye internalisation) in Dap160^Δ1/Dap160^Δ2 mutant synaptic boutons in response to nerve stimulation is significantly reduced compared to controls. Increased uptake is seen when these flies are treated with chlorpromazine.

(Kasprowicz et al., 2008)

Total bouton number at the neuromuscular junction is increased in Dap160^EMS/Dap160^Δ1 larvae compared to controls.

(O'Connor-Giles et al., 2008)

Under high-frequency stimulation conditions (10Hz for 10 minutes), the neuromuscular junctions (NMJs) of Dap160^Δ1/Df(2L)bur-K1 larvae show strong synaptic depression to approximately 40% of the initial excitatory junctional potential (EJP) amplitude (in contrast to control NMJs, which are able to maintain EJPs at approximately 90% of the initial amplitude).

During stimulation, Dap160^Δ1/Df(2L)bur-K1 boutons are seen to contain cisternae and large membraneous bodies that appear to be contiguous with the plasma membrane.

Dap160^Δ1/Df(2L)bur-K1 larvae show reduced dye uptake at the neuromuscular junction compared to controls, indicating a defect in endocytosis.

(Koh et al., 2007)

Dap160^EMS/Dap160^Δ1 adults become paralysed at temperatures between 34^oC and 36^oC.

Dap160^Δ1/Dap160^Δ6 adults show paralysis at 38^oC.

Neuromuscular junction (NMJ) morphology is abnormal in Dap160^Δ1/Dap160^Δ2

third instar larvae; boutons are irregularly spaced and large boutons

are surrounded by smaller satellite boutons. Bouton number normalised

to muscle area is significantly increased in Dap160^Δ1/Dap160^Δ2

larvae (3.5 +/- 0.07 μm^-2) compared to controls (1.7 +/- 0.07 μm^-2).

In addition, the boutons show more frequent branching than controls;

the larval NMJ has 26.2 +/- 2.1 branch points per synapse in Dap160^Δ1/Dap160^Δ2

animals compared to 5.1 +/- 0.3 branch points per synapse for controls.

Neuromuscular junction (NMJ) morphology is abnormal in Dap160^Δ1/Dap160^Δ3

third instar larvae; boutons are irregularly spaced and large boutons

are surrounded by smaller satellite boutons. Extra branches and boutons

are apparent in the mutant larvae.

In 5mM Ca²⁺ at 22^oC, the amplitude of the excitatory junctional

potential (EJP) at the neuromuscular junction (NMJ) in Dap160^Δ1/Dap160^Δ2

or Dap160^Δ1/Dap160^Δ3 third instar larvae is not different

from that of controls. The EJP amplitude at the NMJ of Dap160^Δ1/Dap160^Δ2

third instar larvae is normal in 5mM Ca²⁺ at 34^oC. The EJP amplitude

at the NMJ of Dap160^Δ1/Dap160^Δ3 third instar larvae

is normal in 5mM Ca²⁺ at 36^oC. In 0.5 mM Ca²⁺, the EJP amplitude

at the NMJ of Dap160^Δ1/Dap160^Δ2 third instar larvae

is normal at 22^oC, and is slightly reduced compared to controls at

34^oC.

In 0.5mM Ca²⁺, the frequency of miniature EJPs (mEJPs) at the NMJ

is increased more than 2 fold in Dap160^Δ1/Dap160^Δ2

larvae compared to controls at 22^oC. mEJPs with an unusually large

amplitude are seen at both 22^oC and 34^oC.

In 0.5mM Ca²⁺, the frequency of miniature EJPs (mEJPs) at the NMJ

is increased more than 2 fold in Dap160^Δ1/Dap160^Δ3

larvae compared to controls at 22^oC. mEJPs with an unusually large

amplitude are seen at both 22^oC and 36^oC.

Dap160^Δ1/Dap160^Δ2 and Dap160^Δ1/Dap160^Δ3

larvae show a mild defect in endocytosis at 22^oC (assayed by dye

uptake in third instar fillet preparations), which is exacerbated at

36^oC.

Dap160^Δ1/Dap160^Δ2 larvae show a stimulation-dependent

decrease in EJP amplitude when stimulated at 10Hz. This decrease is

more severe at 34^oC than at 22^oC.

Dap160^Δ1/Dap160^Δ3 larvae show a stimulation-dependent

decrease in EJP amplitude when stimulated at 10Hz. This decrease is

more severe at 36^oC than at 22^oC.

Ultrastructural analysis of the NMJ shows that the number of vesicles

is reduced to about 60% of controls in Dap160^Δ1/Dap160^Δ2

larvae at 22^oC without stimulation. The number of vesicles is further

reduced to about 23% of controls upon stimulation in 60mM K⁺ at 34^oC.

The number of active zones is not reduced in the mutant larvae compared

to controls, but there is a reduction in the number of vesicles at

the active zones in mutant larvae at 22^oC without stimulation and

a further decrease upon stimulation with 60mM K⁺ at 34^oC. There

are an increased number of vesicles with large diameter in the mutant

synapses. Endocytic intermediates, mostly consisting of collared pits

and Ω structures are seen in the mutants (these structures are

rarely seen in controls). The collared pits are seen at both the active

zones and in periactive zones in the mutant larvae.

(Koh et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dap160^Δ1 has abnormal neuroanatomy | larval stage phenotype, suppressible by wit^A12/wit[+]

(O'Connor-Giles et al., 2008)

Enhancer of

Statement

Reference

Dap160[+]/Dap160^Δ1 is an enhancer of abnormal neuroanatomy | larval stage phenotype of nwk¹/nwk²

(O'Connor-Giles et al., 2008)

Suppressor of

Statement

Reference

Dap160[+]/Dap160^Δ1 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dap160^Δ1 has NMJ bouton | increased number | larval stage phenotype, suppressible by wit^A12/wit[+]

(O'Connor-Giles et al., 2008)

Enhancer of

Statement

Reference

Dap160[+]/Dap160^Δ1 is an enhancer of NMJ bouton | larval stage | increased number phenotype of nwk¹/nwk²

(O'Connor-Giles et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Dap160^Δ1/+ enhances the increase in total bouton number and satellite bouton number at the neuromuscular junction that is seen in nwk¹/nwk² larvae.

The increase in total bouton number at the neuromuscular junction that is seen in Dap160^Δ1 larvae is suppressed by wit^A12/+.

(O'Connor-Giles et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dap160^Δ1/Dap160^Δ2 is rescued by Dap160^{+tCH322-154I22}

(Venken et al., 2009)

Dap160^Δ1/Dap160^Δ6 is rescued by Dap160^UAS.cKa/Scer\GAL4^αTub84B.PL

(Koh et al., 2004)

Dap160^Δ1/Dap160^Δ6 is rescued by Scer\GAL4^elav-C155/Dap160^UAS.cKa

(Koh et al., 2004)

Dap160^Δ1/Dap160^Δ3 is rescued by Dap160^UAS.cKa/Scer\GAL4^αTub84B.PL

(Koh et al., 2004)

Dap160^Δ1/Dap160^Δ3 is rescued by Scer\GAL4^elav-C155/Dap160^UAS.cKa

(Koh et al., 2004)

Dap160^Δ1/Dap160^Δ2 is rescued by Dap160^UAS.cKa/Scer\GAL4^αTub84B.PL

(Koh et al., 2004)

Dap160^Δ1/Dap160^Δ2 is rescued by Scer\GAL4^elav-C155/Dap160^UAS.cKa

(Koh et al., 2004)

Dap160^Δ1/Df(2L)bur-K1 is rescued by Scer\GAL4^elav-C155/Dap160^UAS.cKa

(Koh et al., 2004)

Dap160^Δ1/Df(2L)bur-K1 is rescued by Dap160^UAS.cKa/Scer\GAL4^αTub84B.PL

(Koh et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24877

w^*; Dap160^Δ1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

Chromosome probably carries a second lesion, since Dap160^Δ1/Dap160^Δ2 and Dap160^Δ1/Df(2L)bur-K1 animals die later (pharate adult) than Dap160^Δ1 homozygotes (third larval instar).

(Koh et al., 2004)

Comments

Allelic series (based on protein expression data, complementation data and lethal phase): Dap160^Δ1 = Dap160^Δ2 > Dap160^Δ3 = Dap160^Δ4 = Dap160^Δ5 > Dap160^EMS > Dap160^Δ6.

(Koh et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DAP160^Δ1

(Mohrmann et al., 2008)

Dap160^Δ1

(Walker et al., 2013, Kawasaki et al., 2011)

Dap160Δ1

(Spargo et al., 2025)

dap160 Δ1

(Venken, 2006)

dap160^Δ1

(Del Signore et al., 2023, Del Signore et al., 2021, Yao et al., 2017, Winther et al., 2013, Venken et al., 2009, Chabu and Doe, 2008, Kasprowicz et al., 2008, O'Connor-Giles et al., 2008, Koh et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (15)

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