FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Dap160Q24
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General Information
Symbol
Dmel\Dap160Q24
Species
D. melanogaster
Name
FlyBase ID
FBal0160626
Feature type
allele
Associated gene
Dmel\Dap160
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Marie et al., 2004)
Mutagen
X ray
(Marie et al., 2004)
Progenitor genotype
Cytology
Description

4bp deletion resulting in a frame shift after the G286 codon. This allows the addition of a 100 novel amino acids after G286 and a new stop codon at amino acid 386.

(Marie et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:21,140,686..21,140,689 [-]
Comment:

Approximate location of 4 base deletion, which causes a frameshift after amino acid G286. As a result, 100 novel amino acids are added onto the protein before termination at residue 386.

(Marie et al., 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Dap160Q24/Dap160Δ1 third instar larvae have a reduced number of neuroblasts per brain lobe compared to wild-type controls.

      Progression through mitosis is delayed in Dap160Q24/Dap160Δ1 larval neuroblasts compared to wild-type neuroblasts; the transit from nuclear envelope breakdown to anaphase onset is 13.37 +/- 4.4 minutes in the mutant neuroblasts compared to 7.76 +/- 2.04 minutes in wild-type neuroblasts.

      Homozygous single neuroblast clones which are induced in first instar larvae and allowed to develop for 96 hours always contain a single large dpn-positive neuroblast (as do control single neuroblast clones).

      (Chabu and Doe, 2008)

      Mutant larvae are sluggish and stop moving in late larval stages prior to lethality at midlarval stages. Muscle recordings on muscle 6 in abdominal segment A3 of third instar larvae show that in 2mM calcium, the initial EPSP amplitude in Dap160Q24/Df(2L)10523-35 mutants is comparable to that of controls. However, during prolonged stimulus of the synapse, the Dap160Q24/Df(2L)10523-35 mutant synapse shows a monotonic decline in the average EPSP amplitude (in contrast to control synapses, which can sustain evoked release without decrement for 4 minutes). Muscle recordings on muscle 6 in abdominal segment A3 of third instar larvae show that in 0.5mM extracellular calcium, the average mEPSP amplitude is increased by about 60% in Dap160Q24/Df(2L)10523-35 mutants compared to controls. In addition, there is a slight but statistically significant reduction in the average EPSP amplitude in Dap160Q24/Df(2L)10523-35 mutants compared to controls. Quantal content is decreased in Dap160Q24/Df(2L)10523-35 mutants compared to controls. Homozygous and Dap160Q24/Df(2L)10523-35 third instar larvae show an increase in satellite bouton number at the neuromuscular junction compared to controls.

      (Marie et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      D. Huen

      (Marie et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dap160Q24
      dap160Q24
      (Chabu and Doe, 2008, Marie et al., 2004)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)