FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Dap160UAS.cMa
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General Information
Symbol
Dmel\Dap160UAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Marie
FlyBase ID
FBal0160624
Feature type
allele
Associated gene
Dmel\Dap160
Associated Insertion(s)
Carried in Construct
P{UAS-Dap160.M}
Also Known As
UAS-dap160
Key Links
Transgenic product class
cDNA
(Marie et al., 2004)
wild_type
(Marie et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Marie et al., 2004)
wild_type
(Marie et al., 2004)
Mutagen
in vitro construct
(Marie et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-Dap160.M}
Cytology
Description

UASt regulatory sequences drive expression of a full length Dap160 cDNA.

(Marie et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Dap160
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Down syndrome
      CEA with sraUAS.Tag:HA, Synj+tKa
      (Chang and Min, 2009)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      larval neuroblast & larval brain, with Scer\GAL4wor.PA

      (Chabu and Doe, 2008)
      Detailed Description
      Statement
      Reference

      Second instar larvae expressing Dap160Scer\UAS.cMa under the control of Scer\GAL4wor.PA have an increased number of neuroblasts per brain lobe compared to wild-type controls.

      Third instar larvae expressing Dap160Scer\UAS.cMa under the control of Scer\GAL4wor.PA have an reduced number of neuroblasts per brain lobe compared to wild-type controls.

      (Chabu and Doe, 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The evoked excitatory postsynaptic potential (EPSP) amplitude during a 5 minute stimulation at 10 Hz shows a significantly steeper decline in triple transgenic synj+tKa Dap160Scer\UAS.cMa sraScer\UAS.cCa Scer\GAL4elav.PLu flies compared to controls. (This effect is not seen when double or single transgenes are expressed.) No significant difference is seen in miniature EPSP frequency or amplitude between the triple transgenics and controls.

      Dye uptake/unloading assays demonstrate that neuromuscular junctions of triple transgenic synj+tKa Dap160Scer\UAS.cMa sraScer\UAS.cCa Scer\GAL4elav.PLu larvae show a delay in the rate of vesicle uptake, generating an overall defect in endocytosis. Exocytosis and vesicle pool size are unaffected.

      Triple transgenic synj+tKa Dap160Scer\UAS.cMa sraScer\UAS.cCa Scer\GAL4elav.PLu larvae show significantly impaired locomotor activity compared to controls. Triple transgenic adult flies fall more frequently and fail to climb the sides of the vial when mechanical stress is applied - their movement becomes normal after some recovery time.

      Neuromuscular junctions of triple transgenic synj+tKa Dap160Scer\UAS.cMa sraScer\UAS.cCa Scer\GAL4elav.PLu larvae exhibit a ~2.5-fold increase in small satellite boutons. Double transgenic synj+tKa sraScer\UAS.cCa Scer\GAL4elav.PLu larvae show a similar phenotype, though double transgenic synj+tKa Dap160Scer\UAS.cMa Scer\GAL4elav.PLu or Dap160Scer\UAS.cMa sraScer\UAS.cCa Scer\GAL4elav.PLu do not.

      Neuromuscular junctions of triple transgenic synj+tKa Dap160Scer\UAS.cMa sraScer\UAS.cCa Scer\GAL4elav.PLu larvae exhibit an increase in number but a decrease in size of synaptic boutons.

      (Chang and Min, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Dap160Scer\UAS.cMa
      Dap160UAS.cMa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Marie
      (Marie et al., 2004)
      Secondary FlyBase IDs
        References (3)