FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\holn1c07150
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General Information
Symbol
Dmel\holn1c07150
Species
D. melanogaster
Name
FlyBase ID
FBal0161306
Feature type
allele
Associated gene
Dmel\holn1
Associated Insertion(s)
PBac{PB}holn1c07150
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
piggyBac activity
(Bloomington Drosophila Stock Center, 2004.11.6)
Progenitor genotype
Associated Insertion(s)
PBac{PB}holn1c07150
Cytology
Description

PBac{PB} insertion after nucleotide 878 of the holn1 ORF, resulting in a missense mutation causing a K to N switch at amino acid 293 and immediately followed by a stop codon likely truncating the C-terminal GYF domain.

(Geiger et al., 2011)
Allele components
Component
Use(s)
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous embryos that survive to hatching larvae stage are defective in wound healing, though 15% of homozygotes die before hatching. holn1LL07287/holn1c07150 embryos that survive to hatching larvae stage display a similar wound-healing phenotype, though 25% of homozygotes die before hatching.

      Wounded holn1c07150 embryos assemble an actomyosin contractile cable within minutes of wounding, similar to wild type. Actin-containing cell protrusions also form as normal. However, soon after the actomyosin cable has formed, the wound closure process slows down in mutants compared to controls.

      Rare homozygous escapers display a subtle rough eye phenotype and often have extra and/or misplaced or missing macrochaetae on the scutellum.

      (Geiger et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      holn1c07150/CG5198[+] is an enhancer of eye phenotype of Ras85DV12.sev

      (Geiger et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      holn1c07150/+ enhances the Ras85DV12.sev rough eye phenotype.

      (Geiger et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      holn1c07150 is rescued by holn1UASp.cGa/Scer\GAL4e22c

      (Geiger et al., 2011)
      Comments

      Scer\GAL4e22c-mediated expression of holn1Scer\UAS.P\T.cGa rescues the wound healing phenotype of holn1c07150 embryos.

      (Geiger et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Precise excision of the PBac{PB} element reverts the wound healing phenotype and lethality of holn1c07150 animals.

      (Geiger et al., 2011)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG5198c07150
      holn1c07150
      Name Synonyms
      Secondary FlyBase IDs
        References (3)