FlyBase Allele Report: Dmel\gny[f04215]

General Information

Symbol

Dmel\gny^f04215

Species

D. melanogaster

Name

FlyBase ID

FBal0161329

Feature type

allele

Associated gene

Dmel\gny

Associated Insertion(s)

PBac{WH}gny^f04215

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}gny^f04215

Cytology

Description

The PBac{WH}gny^f04215 insertion truncates the gny protein by 20%, including the C-terminal sequences of the glycosyltransferase domain (residues 12-465).

(Shaik et al., 2011)

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:10,381,214..10,381,214 [-]

(Shaik et al., 2011, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of second instar larval stage

(Shaik et al., 2011)

lethal - all die before end of second instar larval stage (with Df(2L)BSC111)

(Shaik et al., 2011)

some die during second instar larval stage

(Shaik et al., 2011)

some die during second instar larval stage (with Df(2L)BSC111)

(Shaik et al., 2011)

Phenotype Manifest In

cephalopharyngeal skeleton | germline clone

(Shaik et al., 2011)

denticle | germline clone

(Shaik et al., 2011)

embryonic/first instar larval cuticle | germline clone

(Shaik et al., 2011)

Detailed Description

Statement

Reference

Mutant embryos have defects in germband extension.

(Zhang et al., 2014)

Homozygous or gny^f04215/Df(2L)BSC6 larvae hatch and have a wild type cuticle by light microscopy. They eventually die after one moult without displaying any obvious phenotype.

Removal of maternal gny in gny^f04215 germline clones results in larvae with patterning and morphological defects, the most prominent being the failure to form a normal head skeleton. The cuticle is discontinuous and the body contours are irregular. At the edges of ruptures, the cuticle is often melanized. Specialized cuticle structures such as denticles are present.

Removal of maternal and zygotic gny in gny^f04215 germline clones causes additional reduction in cuticle deposition and a complete failure of denticle formation and melanization.

(Shaik et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

gny^f04215, wol² has lethal | second instar larval stage phenotype

(Shaik et al., 2011)

Phenotype Manifest In

Other

Statement

Reference

gny^f04215, wol¹ has embryonic/first instar larval cuticle | germline clone phenotype

(Shaik et al., 2011)

gny^f04215, wol¹ has cephalopharyngeal skeleton | germline clone phenotype

(Shaik et al., 2011)

gny^f04215, wol¹ has denticle | germline clone phenotype

(Shaik et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

gny^f04215, wol² double mutant embryos fully develop into larvae that hatch and die at the second instar. Embryos derived from gny^f04215, wol¹ double mutant germline clones show the same cuticle phenotypes as embryos produced from either single mutant germline clone.

(Shaik et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

18744

w¹¹¹⁸; PBac{WH}gny^f04215/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG5091^f04215

alg6^P

(Shaik et al., 2011)

gny^f04215

(Zhang et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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