FB2026_02 , released June 18, 2026
Allele: Dmel\Nlg3f00777
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General Information
Symbol
Dmel\Nlg3f00777
Species
D. melanogaster
Name
FlyBase ID
FBal0161634
Feature type
allele
Associated gene
Dmel\Nlg3
Associated Insertion(s)
PBac{WH}Nlg3f00777
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Xing et al., 2014)
Mutagen
piggyBac activity
(Bloomington Drosophila Stock Center, 2004.11.6)
Progenitor genotype
Associated Insertion(s)
PBac{WH}Nlg3f00777
Cytology
Description
Allele components
Component
Use(s)
Inserted element
PBac{WH}
Regulatory region(s)
UAS
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Excitatory junctional potential (EJP) amplitude and miniature excitatory junctional potential (mEJP) frequency are significantly reduced at the Nlg3KO88/Nlg3f00777 third larval instar neuromuscular junction compared to wild type. mEJP amplitude is not significantly affected.

      Nlg3KO88/Nlg3f00777 and Nlg3KO127/Nlg3f00777 third larval instar larvae show a significant increase in bouton number and a significant decrease in bouton size at the neuromuscular junction compared to wild type. Synaptic branch number at the neuromuscular junction is not significantly affected.

      Ultrastructurally, the neuromuscular junction of Nlg3KO88/Nlg3f00777 larvae shows a number of defects compared to wild type. There is a shift in the distribution of synaptic vesicle diameter to larger values and the number of large vesicles greater than 80nm in diameter is dramatically increased. The number of vesicles at the active zone is significantly decreased, and the postsynaptic density length is significantly increased.

      Synaptic endocytosis at the neuromuscular junction is impaired in Nlg3KO88/Nlg3f00777 larvae.

      (Xing et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      CG32465f00777
      CG34127f00777
      Nlg3f00777
      dnlg3f00777
      (Xing et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)