FlyBase Allele Report: Dmel\ATP8B[f05203]

General Information

Symbol

Dmel\ATP8B^f05203

Species

D. melanogaster

Name

FlyBase ID

FBal0162229

Feature type

allele

Associated gene

Dmel\ATP8B

Associated Insertion(s)

PBac{WH}ATP8B^f05203

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ha et al., 2014)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}ATP8B^f05203

Cytology

Description

PBac insertion in the coding region and predicted to affect all ATP8B isoforms.

(Liu et al., 2014)

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:12,072,449..12,072,449 [+]

(Ha et al., 2014, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal olfactory receptor neuron

(Liu et al., 2014)

Detailed Description

Statement

Reference

ATP8B^f05203 mutant flies display reduced adult survival rate.

(Danilov et al., 2015)

ATP8B^f05203 mutants show defects in cVA (11-cis-vaccenyl acetate) perception.

Expression of Scer\GAL4^Or67d-GAL4-2>ATP8B^{E351Q.Scer\UAS} fails to rescue the defects in cVA (11-cis-vaccenyl acetate) perception of ATP8B^f05203 mutants.

(Ha et al., 2014)

ATP8B^f05203 homozygous or ATP8B^ll2/ATP8B^f05203 trans-heterozygous flies show a reduced electroantennogram response to most tested odorants known to excite a variety of different ORN classes. The response to CO[[2]] is unaffected however.

ATP8B^f05203 homozygous flies show a reduced electroantennogram response to odorants, over a range of concentrations, specifically in Or-expressing olfactory receptor neurons (ORNs), and not in IR- or GR-expressing ORNs. Consistent with these observations, responses from gustatory neurons on the labellum to the tastants sucrose and caffeine is unaffected in ATP8B^f05203 flies.

No difference is observed in the length or shape of the dendrites in antennal sections from ATP8B^f05203 mutants.

(Liu et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ATP8B^f05203 has abnormal neurophysiology phenotype, suppressible by Scer\GAL4^elav.PU/Or67d^UAS.EGFP

(Ha et al., 2014)

ATP8B^f05203 has abnormal smell perception phenotype, suppressible by Scer\GAL4^elav.PU/Or67d^UAS.EGFP

(Ha et al., 2014)

ATP8B^f05203 has abnormal neurophysiology phenotype, suppressible by Scer\GAL4^Or67d-GAL4-2/Btau\ATP8A2^UAS.cHa

(Ha et al., 2014)

Enhancer of

Statement

Reference

ATP8B^f05203 is an enhancer of abnormal neurophysiology phenotype of Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2

(Ha et al., 2014)

ATP8B^f05203 is an enhancer of abnormal smell perception phenotype of Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2

(Ha et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Responses to farnesol are moderately reduced in Scer\GAL4^Or67d-GAL4-2>Or83c^Scer\UAS.cRa transgenic flies in an ATP8B^f05203 mutant background compared with transgenic expression in a wild-type genetic background.

Overexpression of Or67d^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav.PU restores cVA pheromone detection in ATP8B^f05203 mutants.

(Ha et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Btau\ATP8A2^Scer\UAS.cHa under the control of Scer\GAL4^Or67d-GAL4-2 completely suppresses the ATP8B^f05203 mutant cVA-perception defects.

(Ha et al., 2014)

Complementation and Rescue Data

Rescued by

ATP8B^f05203 is rescued by Scer\GAL4^Or67d-GAL4-2/ATP8B^UAS.cHa

(Ha et al., 2014)

ATP8B^f05203 is rescued by ATP8B^UAS.cHb/Scer\GAL4^Or67d-GAL4-2

(Ha et al., 2014)

Not rescued by

ATP8B^f05203 is not rescued by ATP8B^E351Q.UAS/Scer\GAL4^Or67d-GAL4-2

(Ha et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

18847

w¹¹¹⁸; PBac{WH}ATP8B^f05203

Notes on Origin

Discoverer

Comments

Precise excision of the PBac element reverts the aberrant electroantennogram response phenotype.

(Liu et al., 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

ATP8B^f05203

CG14741^f05203

(Zhou et al., 2015)

atg8b¹

(Zhou et al., 2015)

dATP8B⁵

(Ha et al., 2014)

dATP8B^f05203

(Liu et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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