FlyBase Allele Report: Dmel\ema[f07675]

General Information

Symbol

Dmel\ema^f07675

Species

D. melanogaster

Name

FlyBase ID

FBal0162434

Feature type

allele

Associated gene

Dmel\ema

Associated Insertion(s)

PBac{WH}ema^f07675

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Kim et al., 2010)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}ema^f07675

Cytology

Description

PBac{WH}ema^f07675 is inserted within a coding exon of ema.

(Kim et al., 2010)

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,440,064..16,440,103 [+]

(Kim et al., 2010, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | recessive

(Kim et al., 2010)

lethal - all die before end of P-stage | recessive

(Kim et al., 2010)

some die during P-stage | recessive

(Kim et al., 2010)

Phenotype Manifest In

endosome

(Kim et al., 2010)

neuromuscular junction (with Df(3R)Exel7330)

(Kim et al., 2010)

terminal bouton

(Kim et al., 2010)

terminal bouton (with Df(3R)Exel7330)

(Kim et al., 2010)

Detailed Description

Statement

Reference

Animals homozygous for ema^f07675 have an approximately twofold increase in both the number of synaptic boutons and the synaptic area compared with wild-type. ema^f07675/Df(3R)Exel7330 mutants show a very similar phenotype.

ema^f07675 mutants have a dramatically enlarged endosomal compartment and display severe defects in endosomal trafficking.

(Kim et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ema^f07675 has lethal | recessive | P-stage phenotype, suppressible by Hsap\CLEC16A^UAS.GFP/Scer\GAL4^da.G32

(Kim et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

ema^f07675 has terminal bouton phenotype, suppressible | partially by Mad[+]/Mad^1-2

(Kim et al., 2010)

ema^f07675 has endosome phenotype, suppressible by Hsap\CLEC16A^UAS.GFP/Scer\GAL4^da.G32

(Kim et al., 2010)

Enhancer of

Statement

Reference

ema^f07675/CG12753[+] is an enhancer of endosome phenotype of Scer\GAL4^da.G32, Vps16A^RNAi.UAS.WIZ

(Kim et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Mad^1-2/+ partially suppresses the synaptic bouton number phenotype of homozygous ema^f07675 mutants.

ema^f07675/+ enhances the endosomal phenotype observed in cell expressing Vps16A^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^da.G32, as there is an additional twofold increase in the size of endosomes.

(Kim et al., 2010)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\CLEC16A^Scer\UAS under the control of Scer\GAL4^da.G32 rescues the lethality and the endosomal phenotypes associated with ema^f07675 mutants.

(Kim et al., 2010)

Complementation and Rescue Data

Rescued by

ema^f07675 is rescued by ema^UAS.EGFP/Scer\GAL4^Act5C.PI

(Kim et al., 2010)

ema^f07675/Df(3R)Exel7330 is rescued by ema^UAS.EGFP/Scer\GAL4^elav.PLu

(Kim et al., 2010)

Comments

Expression of ema^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^elav.PLu rescues the pupal lethality and the synaptic phenotype at the neuromuscular junction associated with ema^f07675/Df(3R)Exel7330.

Expression of ema^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^Act5C.PI rescues the endosomal trafficking defects of ema^f07675 mutants.

(Kim et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

19092

w¹¹¹⁸; PBac{WH}ema^f07675/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG12753 f07675

(Kim et al., 2010)

CG12753^f07675

ema¹

(Kim et al., 2010)

ema^f07675

(Lund et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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