50% of nesdEY08338 mutant males are sterile. Males exhibit multinucleate spermatids in 21% of cells. These spermatids display a failure of cytokinesis during one or both meiotic divisions. Onion stage spermatids contain two or four haploid nuclei accompanied by an enlarged Nebenkern.
The contractile ring constricts asymmetrically in 46% of nesdEY08338 mutant spermatocytes. Subsequently, the cleavage furrow collapses and the contractile ring fails to form a robust circular structure. Membrane invagination collapses and isolated contractile ring remnants are observed in the cytoplasm. Cytokinesis fails in 49% of spermatocytes, and plasma membrane invagination is asymmetric and incomplete in 27% of mutant cells. Ring canals fail to assemble during late cytokinesis.
80% of nesdEY08338/Df(2L)ED1305 mutant males are sterile. Males exhibit multinucleate spermatids in 32% of cells. These spermatids display a failure of cytokinesis during one or both meiotic divisions. Onion stage spermatids contain two or four haploid nuclei accompanied by an enlarged Nebenkern.
38% of nesdEY08338 mutant egg chambers at stage 9 and 10 contain remnants of ring canals associated with binucleate nurse cells, which is never observed in control animals or nesdEY08338 egg chambers from an earlier developmental stage.
Df(2L)ED1305/nesdEY08338 has abnormal meiotic cell cycle phenotype, enhanceable by pav[+]/pavB200
Df(2L)ED1305/nesdEY08338 has male semi-sterile phenotype, suppressible by pavUbi-p63E.mGFP6
Df(2L)ED1305/nesdEY08338 has abnormal meiotic cell cycle phenotype, suppressible by pavUbi-p63E.mGFP6
Df(2L)ED1305/nesdEY08338 has partially lethal - majority die phenotype, non-suppressible by pavUbi-p63E.mGFP6
Expression of pavUbi-p63E.T:Avic\GFP-m6 fully rescues the cytokinesis and sterility defects of nesdEY08338/Df(2L)ED1305 mutant flies, but not the lethality of the mutants.
nesdEY08338/Df(2L)ED1305 mutant flies with one copy of pavB200 show a two-fold increase in then number of multinucleate spermatids.
nesdEY08338 is rescued by nesdC.Ubi.GFP
nesdEY08338 is not rescued by nesdN.Ubi.GFP
nesdEY08338 is not rescued by nesd1-292.C.Ubi.GFP
nesdEY08338 is not rescued by nesd1-292.N.Ubi.GFP
Expression of nesdC.Ubi.T:Avic\GFP rescues both lethality and the cytokinesis defects of nesdEY08338 mutant flies.
Expression of nesdN.Ubi.T:Avic\GFP fails to rescue both lethality and the cytokinesis defects of nesdEY08338 mutant flies.
Expression of nesd1-292.C.T:Avic\GFP fails to rescue both lethality and the cytokinesis defects of nesdEY08338 mutant flies.
Expression of nesd1-292.N.T:Avic\GFP fails to rescue both lethality and the cytokinesis defects of nesdEY08338 mutant flies.